Nelson J C, Tomei R T
Department of Pathology, White Memorial Medical Center, Los Angeles, CA 90033.
Clin Chem. 1988 Sep;34(9):1737-44.
We have designed a re-usable dialysis cell and a complex dialysis buffer, with which undiluted serum samples can be dialyzed with minimal changes in their serum matrix. Dialysate thyroxin (free T4) is then measured by a sensitive RIA for T4. The range of reportability was 2-128 ng/L, the normal range was 8-27 ng/L, and the interassay CV was 7%. Free T4 concentrations in various disorders were as follows: hyperthyroidism, 32-478 ng/L; in both excess thyroxin-binding globulin (TBG) and familial dysalbuminemic hyperthyroxinemia, 9-27 ng/L; primary hypothyroidism, less than 2-7 ng/L; central hypothyroidism, 4-6 ng/L; severe TBG deficiency, 9-25 ng/L; hypothyroxinemias of nonthyroidal illness, 8-35 ng/L. With this free-T4 assay, which is adaptable to clinical laboratory use, one can differentiate hyperthyroidism from the major euthyroid hyperthyroxinemias and hypothyroidism from the major euthyroid hypothyroxinemias.
我们设计了一种可重复使用的透析池和一种复合透析缓冲液，利用它们可以对未稀释的血清样本进行透析，且血清基质变化极小。然后通过一种灵敏的甲状腺素放射免疫分析法（RIA）来测定透析液中的甲状腺素（游离T4）。可报告范围为2 - 128 ng/L，正常范围为8 - 27 ng/L，批间变异系数（CV）为7%。各种疾病状态下的游离T4浓度如下：甲状腺功能亢进症，32 - 478 ng/L；甲状腺素结合球蛋白（TBG）增多症和家族性异常白蛋白血症性甲状腺素增多症，9 - 27 ng/L；原发性甲状腺功能减退症，低于2 - 7 ng/L；中枢性甲状腺功能减退症，4 - 6 ng/L；严重TBG缺乏症，9 - 25 ng/L；非甲状腺疾病引起的甲状腺素减少症，8 - 35 ng/L。通过这种适用于临床实验室的游离T4检测方法，可以区分甲状腺功能亢进症与主要的甲状腺功能正常的甲状腺素增多症，以及甲状腺功能减退症与主要的甲状腺功能正常的甲状腺素减少症。
