2'-脱氧鸟苷处理的小鼠胚胎胸腺在裸鼠中的肾被膜下移植

Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice.

作者信息

Wang Jianxue, Chen Guolin, Cui Qianwen, Song Erfei, Tao Weihua, Chen Wanqun, Wang Cunchuan, Jia Shiqi

机构信息

The First Affiliated Hospital, Jinan University; The Institute of Clinical Medicine, Jinan University; Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong.

Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Jinan University; The Institute of Clinical Medicine, Jinan University; Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong.

出版信息

J Vis Exp. 2019 Jul 19(149). doi: 10.3791/59657.

DOI:10.3791/59657

PMID:31380836

Abstract

The thymus is an important central immune organ, which plays an essential role in the development and differentiation of T cells. Thymus transplantation is an important method for investigating thymic epithelial cell function and T cells maturation in vivo. Here we will describe the experimental methods used within our laboratory to transplant 2'-deoxyguanosine (to deplete donor's lymphocytes) treated embryonic thymus into the renal capsule of an athymic nude mouse. This method is both simple and efficient and does not require special skills or devices. The results obtained via this simple method showed that transplanted thymus can effectively support the recipient's T cells production. Additionally, several key points with regards to the protocol will be further elucidated.

摘要

胸腺是一个重要的中枢免疫器官，在T细胞的发育和分化中起着至关重要的作用。胸腺移植是在体内研究胸腺上皮细胞功能和T细胞成熟的重要方法。在这里，我们将描述我们实验室用于将经2'-脱氧鸟苷（用于耗尽供体淋巴细胞）处理的胚胎胸腺移植到无胸腺裸鼠肾被膜内的实验方法。该方法既简单又高效，不需要特殊技能或设备。通过这种简单方法获得的结果表明，移植的胸腺可以有效地支持受体T细胞的产生。此外，将进一步阐明该方案的几个关键点。