Sander C, Hadwiger L A
Biochim Biophys Acta. 1979 Jul 26;563(2):278-92. doi: 10.1016/0005-2787(79)90047-9.
The substitution of the base analogue 5-bromodeoxyuridine (BrdU) for thymidine in the DNA of pea pods (Pisum sativum) induces or enhances the level of phenylalanine ammonia-lyase (PAL) and also induces the phytoalexin, pisatin, a product of the same metabolic pathway. Cordycepin, a polyadenylate inhibitor at the RNA level, is a potent inhibitor of pisatin synthesis. Kinetic studies on the inhibition of the PAL-pisatin production by hydroxyurea indicate that BrdU must be incorporated into DNA before any induction takes place. 5-Iododeoxyuridine is also an inducer while 5-fluorodeoxyuridine is ineffective when applied alone. BrdU is incorporated into the DNA of pea cells and the nuclei undergoes condensation just prior to the detection of the induced responses.
用碱基类似物5-溴脱氧尿苷（BrdU）替代豌豆荚（豌豆）DNA中的胸腺嘧啶核苷，可诱导或提高苯丙氨酸解氨酶（PAL）的水平，还可诱导植保素豌豆素（pisatin），它是同一代谢途径的产物。虫草素是RNA水平的聚腺苷酸抑制剂，是豌豆素合成的有效抑制剂。关于羟基脲对PAL-豌豆素产生抑制作用的动力学研究表明，在任何诱导发生之前，BrdU必须掺入DNA中。5-碘脱氧尿苷也是一种诱导剂，而单独应用5-氟脱氧尿苷则无效。BrdU掺入豌豆细胞的DNA中，并且在检测到诱导反应之前细胞核会发生凝聚。
