Shepherd G W, Flickinger R
Biochim Biophys Acta. 1979 Jul 26;563(2):413-21. doi: 10.1016/0005-2787(79)90060-1.
The control of mRNA diversity during frog development has been investigated. Nuclear and messenger RNA from the early neurula and larval stages of Rana pipiens were hybridized in vast excess to labeled single-copy DNA and resistance to S1 nuclease was measured. Mixtures of RNA populations were also hybridized with single-copy DNA as a measurement of sequence overlap. Neurula and larval nuclear RNA hybridize to 11.3% and 12.1% of the single copy DNA. A mixture of both nuclear RNA populations hybridizes to 10.8% of the DNA, indicating a great amount of sequence overlap between the two populations. The mRNA complexity almost doubles during this developmental period from 4.7% of the single-copy DNA complexity at the early neurula to 8.7% at the larval stage. Mixtures of nuclear and messenger RNA were used to hybridize single-copy DNA and the results indicate that mRNA sequences present on neurula polysomes, but not on larval polysomes, are found in larval nuclei. Furthermore, mRNA sequences found on larval polysomes, but not on neurula polysomes, are found in the neurula nuclei. The data indicate that post-transcriptional events appear to play a role in the qualitative control of mRNA diversity during development.
对青蛙发育过程中mRNA多样性的控制进行了研究。将豹蛙早期神经胚和幼虫阶段的核RNA和信使RNA与标记的单拷贝DNA大量杂交，并测定对S1核酸酶的抗性。RNA群体的混合物也与单拷贝DNA杂交，作为序列重叠的一种测量方法。神经胚和幼虫核RNA分别与11.3%和12.1%的单拷贝DNA杂交。两种核RNA群体的混合物与10.8%的DNA杂交，表明这两个群体之间存在大量的序列重叠。在这个发育阶段，mRNA复杂性几乎增加了一倍，从早期神经胚阶段单拷贝DNA复杂性的4.7%增加到幼虫阶段的8.7%。用核RNA和信使RNA的混合物与单拷贝DNA杂交，结果表明，在神经胚多聚核糖体上存在但在幼虫多聚核糖体上不存在的mRNA序列，在幼虫细胞核中被发现。此外，在幼虫多聚核糖体上存在但在神经胚多聚核糖体上不存在的mRNA序列，在神经胚细胞核中被发现。数据表明，转录后事件似乎在发育过程中mRNA多样性的定性控制中发挥作用。
