Paolucci M, Botte V
Dipartimento di Zoologia, Università di Napoli, Italy.
Gen Comp Endocrinol. 1988 Jun;70(3):466-76. doi: 10.1016/0016-6480(88)90123-2.
Estradiol-binding molecules have been found both in cytosol and nuclear extract of hepatocytes of the female green frog Rana esculenta. These molecules show the properties of an estradiol receptor (Re): high and specific affinity for estrogens (2.10-7.10 x 10(-10) M), stability of binding at 0 and 20 degrees, localization in the nucleus, and cytosolic versus nuclear binding shift in estradiol-treated frogs. In hepatocytes, both filled and unfilled Re is detectable in all stages of the R. esculenta annual cycle. It increases during the recovery phase (September to January) when vitellogenetic processes are active in the ovary. These changes are positively correlated with vitellogenin. Only nuclear filled Re, moreover, is positively correlated to level of circulating estradiol. This suggests a direct connection: plasma estradiol level versus nuclear filled Re versus plasma vitellogenin level.
在雌性食用蛙（Rana esculenta）肝细胞的胞质溶胶和核提取物中均发现了雌二醇结合分子。这些分子表现出雌二醇受体（Re）的特性：对雌激素具有高亲和力和特异性亲和力（2.10 - 7.10×10⁻¹⁰ M），在0℃和20℃时结合稳定，定位于细胞核，并且在经雌二醇处理的青蛙中存在胞质溶胶与细胞核结合的转移。在肝细胞中，在食用蛙年度周期的所有阶段均可检测到填充和未填充的Re。在恢复阶段（9月至1月），当卵巢中的卵黄生成过程活跃时，它会增加。这些变化与卵黄蛋白原呈正相关。此外，只有细胞核填充的Re与循环雌二醇水平呈正相关。这表明存在直接联系：血浆雌二醇水平与细胞核填充的Re与血浆卵黄蛋白原水平之间的联系。
