The Second Dental Center of Peking University School of Stomatology, Beijing 100101, China.
Department of Stomatology, Peking University First Hospital, Beijing 100034, China.
J Nanosci Nanotechnol. 2020 Feb 1;20(2):1046-1055. doi: 10.1166/jnn.2020.16940.
Silver nanoparticles can be used to effectively disinfect in dental root canal therapy, of which the mechanism remains unclear. In the current study, we explored the effect of silver nanoparticles on bacterial growth and changes in the transcriptome of using RNA-sequencing technology. Results showed that silver nanoparticles significantly inhibited growth of by prolonging the lag phase in a dose dependent manner. A total of 100 differentially expressed genes (DEGs) including 3 upregulated and 97 downregulated DEGs were obtained in after treatment with silver nanoparticles. We performed Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses on these DEGs. Functional annotation of DEGs showed that transport and metabolism, such as carbohydrate metabolic process, peptide transport, and amide transport were most significantly enriched. Dysregulated genes were significantly enriched in pathways related to carbohydrate and energy metabolism, and the TCA cycle may be a major mechanism for with silver nanoparticle treatment. These data indicated changes in the transcriptome of after silver nanoparticle treatment, thus providing a mechanism for supporting application of silver nanoparticles for disinfection of during and after root canal therapy.
银纳米颗粒可有效用于根管治疗中的消毒，但其作用机制尚不清楚。在本研究中，我们使用 RNA 测序技术探讨了银纳米颗粒对细菌生长和转录组变化的影响。结果表明，银纳米颗粒通过延长迟滞期，呈剂量依赖性显著抑制的生长。在银纳米颗粒处理后，获得了 100 个差异表达基因（DEGs），包括 3 个上调和 97 个下调 DEGs。我们对这些 DEGs 进行了基因本体（GO）富集和京都基因与基因组百科全书（KEGG）途径分析。DEGs 的功能注释表明，转运和代谢过程，如碳水化合物代谢过程、肽转运和酰胺转运等，最为显著富集。失调基因在与碳水化合物和能量代谢相关的途径中显著富集，三羧酸（TCA）循环可能是银纳米颗粒处理后细菌的主要作用机制。这些数据表明，银纳米颗粒处理后细菌的转录组发生了变化，从而为银纳米颗粒在根管治疗期间和之后用于消毒提供了机制支持。
