Zhang Fu-hua, Li Mao, Wei Zhi-jun, Zhao Bing
Department of Endodontics, Hefei Stomatological Hospital. Hefei 230001, Anhui Province, China. E-mail:
Department of Endodontics, Hefei Stomatological Hospital. Hefei 230001, Anhui Province, China.
Shanghai Kou Qiang Yi Xue. 2016 Feb;25(1):11-5.
To evaluate the efficacy of Ca(OH)2 with a silver nanoparticle suspension to eliminate the biofilm of Enterococcus faecalis (E. faecalis) in starvation phase.
The biofilm models of E．faecalis in the starvation phase were built in vitro with 256 extracted human single-rooted teeth, and the methods of plate culture count and crystal violet biofilm assay were applied to determine the effect of the inhibition of different intracanal medicament (silver nanoparticle with calcium hydroxide, silver nanoparticle alone, calcium hydroxide alone ) to the biofilm of E. faecalis in starvation phase at 1 and 7 days. The negative control group was treated with sterile water only. Statistical analysis was carried out with SPSS 13.0 software package.
The inhibitory effect of Ca（OH）2+nanosilver on the biofilms of E. faecalis was found more significant than that of silver nanoparticle alone and calcium hydroxide alone at 1 and 7 days, and silver nanoparticle alone was more effective than calcium hydroxide alone. No difference in antimicrobial properties was observed between the two time points in the Ca(OH)2+silver nanoparticle group and silver nanoparticle group, while higher antimicrobial efficacy was observed in the Ca(OH)2 group after 7 days than 1 day.
Silver nanoparticle with calcium hydroxide has an obvious inhibitory effect on the biofilm of E.faecalis in the starvation phase.
评估氢氧化钙与纳米银混悬液消除粪肠球菌饥饿期生物膜的效果。
用256颗拔除的人单根牙体外构建粪肠球菌饥饿期生物膜模型，采用平板培养计数法和结晶紫生物膜检测法，分别于1天和7天测定不同根管内药物（纳米银与氢氧化钙、单纯纳米银、单纯氢氧化钙）对饥饿期粪肠球菌生物膜的抑制作用。阴性对照组仅用无菌水处理。采用SPSS 13.0软件包进行统计学分析。
1天和7天时，发现氢氧化钙+纳米银对粪肠球菌生物膜的抑制作用比单纯纳米银和单纯氢氧化钙更显著，且单纯纳米银比单纯氢氧化钙更有效。氢氧化钙+纳米银组和纳米银组在两个时间点的抗菌性能无差异，而氢氧化钙组7天时的抗菌效果高于1天时。
氢氧化钙与纳米银对饥饿期粪肠球菌生物膜有明显抑制作用。
