Rhoptry 蛋白 16（ROP16）改变人 293T 细胞中的细胞凋亡。
Rhoptry Protein 16 (ROP16) Modifies Apoptosis in Human 293T Cells.
机构信息
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, MOH, China; National Center for International Research on Tropical Diseases, China; WHO Collaborating Center for Tropical Diseases, Shanghai 200025, PR China.
Department of Clinical Laboratory, The Affiliated Dongtai Hospital of Nantong University, Dongtai 224200, Jiangsu, PR China.
出版信息
J Nanosci Nanotechnol. 2020 Jan 1;20(1):24-30. doi: 10.1166/jnn.2020.16885.
is an obligate intracellular protozoan. tachyzoites can invade nucleated host cells and inhibit their apoptosis. Therefore, the goal of the present study was to determine whether rhoptry protein 16 (ROP16) secreted by the invading can reduce the apoptotic response of the host cell. For this purpose, a vector for overexpression of ROP16 was constructed and used to transfect human 293T cells. Cells transfected with the vector robustly expressed ROP16, as evidenced by Western blotting. Apoptosis of 293T cells was induced by incubation with 0.5 g/mL actinomycin D (ActD) for 24 h, and its magnitude was measured using Annexin V-FITC/PI and TUNEL assays. Cells transfected by ROP16-expressing vector were characterized by a significantly lower level of apoptosis measured by both techniques.Moreover, caspase-3 activity was also reduced. Thus, ROP16 inhibited ActD-induced apoptosis of human 293T cells, documenting the ability of this rhoptry protein to modulate apoptosis of host cells infected by .
是一种专性细胞内原生动物。速殖子可以侵入有核宿主细胞并抑制其细胞凋亡。因此，本研究的目的是确定入侵的 分泌的 rhoptry 蛋白 16（ROP16）是否可以降低宿主细胞的凋亡反应。为此，构建了用于 ROP16 过表达的载体，并用于转染人 293T 细胞。Western blot 证实，转染载体的细胞中 ROP16 表达旺盛。用 0.5 g/mL 放线菌酮 D（ActD）孵育 24 h 诱导 293T 细胞凋亡，并通过 Annexin V-FITC/PI 和 TUNEL 测定法测量其幅度。两种技术测量的凋亡水平均显著降低，表明 ROP16 抑制了 ActD 诱导的人 293T 细胞凋亡。此外，caspase-3 活性也降低。因此，ROP16 抑制了 ActD 诱导的人 293T 细胞凋亡，证明了这种 rhoptry 蛋白能够调节感染 的宿主细胞的凋亡。
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