肌球蛋白诱导的上皮-间充质转化抑制高转移性结直肠癌的生长。

Epithelial-mesenchymal transition induced by MyoD inhibits growth of high metastatic colorectal cancer.

机构信息

Department of General Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, Hubei, China.

Digestive Department, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, Hubei, China.

出版信息

Med Hypotheses. 2019 Sep;130:109285. doi: 10.1016/j.mehy.2019.109285. Epub 2019 Jun 24.

DOI:10.1016/j.mehy.2019.109285

PMID:31383323

Abstract

OBJECTIVES

The study aimed to investigate the tumor-suppressing factor myogenicity differentiation factor (MyoD) against high metastatic colorectal cancer through its powerful transformation by which the tumor cells were converted into muscle cells or other cells to inhibit the malignant proliferation of tumor cells.

METHODS

The roles of MyoD in colon cancer proliferation, invasion and migration were analyzed by CCK-8 assay and Transwell, and EMT by real-time PCR and Western blot. The secretion of TGFβ1 was assayed by ELISA and activation of p-Smad2/3 were assayed by western blot. The effects of MyoD on intestinal cancer growth and EMT in vivo were also analyzed.

RESULTS

We found MyoD inhibited the proliferation, invasion and migration of colon cancer cell. Moreover, MyoD inhibited the expression of E-cadherin and promoted the expression of vimentin and α-SMA. The secretion of TGFβ1 increased and p-Smad2/3 was activated after MyoD expression. MyoD also inhibits intestinal cancer growth and promoted EMT in vivo.

CONCLUSION

Our findings indicate that MyoD inhibited cancer progression and metastasis by promoting EMT through TGF-β1/Smad2/3 activation, which provide new support for MyoD maybe as a novel anti-cancer method for the treatment of colon cancer in the future.

摘要

目的

本研究旨在通过肌源性分化因子（MyoD）的强大转化作用，将肿瘤细胞转化为肌肉细胞或其他细胞，从而抑制肿瘤细胞的恶性增殖，研究 MyoD 对高转移性结直肠癌的肿瘤抑制因子作用。

方法

通过 CCK-8 检测和 Transwell 分析 MyoD 在结肠癌细胞增殖、侵袭和迁移中的作用，通过实时 PCR 和 Western blot 分析 EMT，通过 ELISA 检测 TGFβ1 的分泌，通过 Western blot 检测 p-Smad2/3 的激活。还分析了 MyoD 对体内肠癌生长和 EMT 的影响。