Viral Immunity and Pathogenesis Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Viral Immunity and Pathogenesis Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
J Immunol. 2019 Aug 15;203(4):775-781. doi: 10.4049/jimmunol.1900232.
Scientists have long valued the power of in vivo observation to answer fundamental biological questions. Over the last 20 years, the application and evolution of intravital microscopy (IVM) has vastly increased our ability to directly visualize immune responses as they are occurring in vivo after infection or immunization. Many IVM strategies employ a strong multiphoton laser that penetrates deeply into the tissues of living, anesthetized mice, allowing the precise tracking of the movement of cells as they navigate complex tissue environments. In the realm of viral infections, IVM has been applied to better understand many critical phases of effector T cell responses, from activation in the draining lymph node, to the execution of effector functions, and finally to the development of tissue-resident memory. In this review, we discuss seminal studies incorporating IVM that have advanced our understanding of the biology of antiviral CD8 T cells.
科学家长期以来一直重视活体观察的力量，以回答基本的生物学问题。在过去的 20 年中，活体显微镜技术（IVM）的应用和发展极大地提高了我们直接观察感染或免疫后体内免疫反应的能力。许多 IVM 策略采用强的多光子激光，该激光可以深入穿透活体麻醉小鼠的组织，从而可以精确跟踪细胞在导航复杂组织环境时的运动。在病毒感染领域，IVM 已被用于更好地理解效应 T 细胞反应的许多关键阶段，从引流淋巴结中的激活，到效应功能的执行，最后到组织驻留记忆的发展。在这篇综述中，我们讨论了一些将 IVM 纳入其中的开创性研究，这些研究加深了我们对抗病毒 CD8 T 细胞生物学的理解。
