Beijing Key Laboratory of Diabetes Prevention and Research, Department of Endocrinology, Lu He Hospital, Capital Medical University, Beijing, 101149, China.
Department of Biomedical Informatics, School of Basic Medical Sciences, Center for Noncoding RNA Medicine, Peking University, Beijing, 100191, China.
Sci Rep. 2019 Aug 5;9(1):11347. doi: 10.1038/s41598-019-47726-z.
LADA (latent autoimmune diabetes in adults), a special subtype of type 1 diabetes, turns out to exhibit phenotypes mimicking the type 2 diabetes, which results in serious misdiagnosis issues. In order to better distinguish LADA from other diabetes subtypes, specific diagnostic and prognostic biomarkers of LADA are required. Circulating microRNAs (miRNAs) are recently shown to be promising biomarkers for disease diagnosis and subtyping. In this study, serum samples from LADA patients and type 2 diabetes patients were collected during the first diagnosis of diabetes and the miRNA transcriptomes of these patients and healthy individuals were profiled. Comparative analysis shows that the differentially expressed miRNAs between groups and their predicted target genes are enriched for several functions including immune regulation. Besides, a few miRNAs showing distinct expression pattern in LADA patients could discriminate LADA from type 2 diabetes, as validated by further qRT-PCR assay. In all, our study implies potential miRNA biomarkers which would be investigated in further clinical researches.
LADA（成人隐匿性自身免疫性糖尿病）是 1 型糖尿病的一种特殊亚型，其表现出的表型类似于 2 型糖尿病，这导致了严重的误诊问题。为了更好地区分 LADA 与其他糖尿病亚型，需要寻找 LADA 的特定诊断和预后生物标志物。循环 microRNAs（miRNAs）最近被证明是疾病诊断和亚型分类的有前途的生物标志物。在这项研究中，在糖尿病的首次诊断时收集了 LADA 患者和 2 型糖尿病患者的血清样本，并对这些患者和健康个体的 miRNA 转录组进行了分析。比较分析表明，组间差异表达的 miRNAs 及其预测的靶基因富集了几种功能，包括免疫调节。此外，一些在 LADA 患者中表现出明显表达模式的 miRNA 可以通过进一步的 qRT-PCR 检测来区分 LADA 和 2 型糖尿病，这一结果得到了验证。总之，我们的研究暗示了一些潜在的 miRNA 生物标志物，这些标志物将在进一步的临床研究中进行研究。
