Department of Endocrinology, The Second Hospital of Jilin University, Changchun, China.
Genet Test Mol Biomarkers. 2022 Mar;26(3):118-126. doi: 10.1089/gtmb.2021.0205.
Expression of circular RNAs (circRNAs) in the peripheral blood of individuals with latent autoimmune diabetes in adults (LADA) and type 2 diabetes mellitus (T2DM) were quantified to identify dysregulated circRNAs compared with control individuals. circRNAs were obtained from the peripheral blood serum of 12 healthy adults and 12 individuals with LADA and 12 type 2 diabetics. The circRNA expression profiles were analyzed by high-throughput RNA sequencing. The most highly dysregulated circular RNAs were validated by quantitative real-time polymerase chain reaction. A circular RNA-microRNA (miRNA) network diagram predicted the interactions of circular RNAs, miRNAs, and coding genes. A total of 2334 differentially expressed circRNAs were detected among the three groups, with 277 circRNAs in the Group DM versus Group NG; 992 circRNAs in the Group LADA versus Group NG and 1065 circRNAs in the Group DM versus Group LADA. Six circRNAs were identified as the most distinctive differentially expressed targets ( < 0.05). The proposed molecular functions of these differentially expressed circRNAS included the tumor necrosis factor signaling pathway, the FoxO signaling pathway, cellular senescence, and long-term potentiation (all false discovery rate < 0.05) which may contribute to T2DM and LADA. circRNAs are aberrantly expressed in the peripheral blood of patients with T2DM and LADA and may interact with miRNA and circRNA-derived peptides in the development of diabetes. Further investigations may illustrate the partial pathogenesis of diabetes mellitus. Clinical Trial Registration number: ChiCTR1900020644.
检测成人隐匿性自身免疫性糖尿病（LADA）和 2 型糖尿病（T2DM）患者外周血中的环状 RNA（circRNAs）表达水平，以鉴定与对照组相比失调的 circRNAs。从 12 名健康成年人、12 名 LADA 患者和 12 名 2 型糖尿病患者的外周血血清中获得 circRNAs。通过高通量 RNA 测序分析 circRNA 表达谱。通过定量实时聚合酶链反应验证最显著失调的 circRNAs。circRNA-miRNA（miRNA）网络图预测 circRNAs、miRNAs 和编码基因的相互作用。在三组中检测到 2334 个差异表达的 circRNAs，其中 277 个在 DM 组与 NG 组之间；992 个在 LADA 组与 NG 组之间，1065 个在 DM 组与 LADA 组之间。鉴定出 6 个作为最具特征性的差异表达靶基因（ < 0.05）。这些差异表达 circRNA 的拟议分子功能包括肿瘤坏死因子信号通路、FoxO 信号通路、细胞衰老和长期增强（所有错误发现率 < 0.05），这些可能导致 T2DM 和 LADA。T2DM 和 LADA 患者外周血中 circRNAs 表达异常，可能与 miRNA 和 circRNA 衍生肽在糖尿病发病机制中相互作用。进一步的研究可能阐明糖尿病的部分发病机制。临床试验注册号：ChiCTR1900020644。
