Smilkova Marcela, Smilek Jiri, Kalina Michal, Klucakova Martina, Pekar Miloslav, Sedlacek Petr
1Institute of Physical and Applied Chemistry, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.
2Materials Research Centre, Brno University of Technology, Faculty of Chemistry, Purkynova 464/118, 612 00 Brno, Czech Republic.
Plant Methods. 2019 Jul 31;15:83. doi: 10.1186/s13007-019-0469-x. eCollection 2019.
Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration of humates through plant cuticles is proposed, tested, and evaluated.
The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amount of penetrated humate in a hydrogel diffusion medium. leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested.
The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.
确定供应给植物的液态腐殖酸盐的运输程度和速率对于测试这类通常以叶面喷施形式供应的生物刺激剂的生理效应至关重要。因此，提出、测试并评估了一种用于定性研究和定量描述腐殖酸盐透过植物角质层渗透情况的原创实验方法。
所提出的方法包括分离模型植物叶片角质层以及随后对角质层腐殖酸盐运输进行体外评估。所采用的新方法基于一种简单的扩散偶装置，涉及对水凝胶扩散介质中渗透的腐殖酸盐量进行连续分光光度测定。通过化学和酶处理分离叶片角质层，并随时间从定量和定性方面估计一种商业腐殖酸盐（木质素腐殖酸盐）的角质层渗透速率。还针对不同的角质层提取方法测定了叶片背腹面角质层木质素腐殖酸盐的不同运输速率。
所提出的方法是一种用于研究基于腐殖酸的生物刺激剂经角质层渗透的简单且廉价的实验工具。
