Torosian M H, Miller E E
Department of Surgery, University of Pennsylvania, School of Medicine, Philadelphia 19104.
J Surg Res. 1988 Oct;45(4):376-81. doi: 10.1016/0022-4804(88)90134-5.
Stimulation of tumor and host tissue proliferation occurs with nutritional support of the tumor-bearing host. This study was performed to determine the effect of parenteral nutrition on bone marrow cytokinetics in tumor-bearing animals. Forty-three Lewis/Wistar rats with subcutaneous mammary tumor implants (AC-33) were protein-depleted for 8 days, underwent superior vena cava cannulation and were randomized to receive intravenous saline or total parenteral nutrition (TPN). Animals receiving TPN were sacrificed after 2, 6, 12, 24, and 48 hr and bone marrow cells were harvested for cell cycle analysis by flow cytometry. Compared to control animals, a significant increase in bone marrow cells in S (DNA synthetic) phase was demonstrated after 24 hr of TPN. The ratio of sensitive/resistant bone marrow cells to chemotherapy specific for cells actively synthesizing DNA was increased after 24 hr of TPN. This alteration in bone marrow cytokinetics was transient and distinct from the previously documented stimulation of tumor growth kinetics. The potential therapeutic implications of differentially stimulating host tissue and tumor growth kinetics with TPN are discussed.
对荷瘤宿主给予营养支持会刺激肿瘤和宿主组织增殖。本研究旨在确定肠外营养对荷瘤动物骨髓细胞动力学的影响。43只皮下植入乳腺肿瘤（AC - 33）的Lewis/Wistar大鼠先进行8天的蛋白质消耗，然后进行上腔静脉插管，并随机分为接受静脉注射生理盐水或全肠外营养（TPN）两组。接受TPN的动物在2、6、12、24和48小时后处死，采集骨髓细胞，通过流式细胞术进行细胞周期分析。与对照动物相比，TPN治疗24小时后，处于S（DNA合成）期的骨髓细胞显著增加。TPN治疗24小时后，对活跃合成DNA的细胞具有特异性的化疗敏感/耐药骨髓细胞比例增加。骨髓细胞动力学的这种改变是短暂的，且与先前记录的肿瘤生长动力学刺激不同。文中讨论了用TPN差异性刺激宿主组织和肿瘤生长动力学的潜在治疗意义。
