Schmidt R H, Dubach M D
University of Washington, Department of Neurological Surgery, Seattle 98195.
J Neurosci Methods. 1988 Jul;24(3):243-51. doi: 10.1016/0165-0270(88)90169-0.
Because a unilateral lesion of the dopaminergic nigrostriatal system has been found to induce rotational behavior in primates, as well as in rodents, we have designed and constructed a rotation monitoring device suitable for non-human primates. Animals are connected via a tether to a continuous-rotation potentiometer which provides a position-dependent output voltage. This voltage is interfaced via an analog-to-digital converter to an IBM PC. The rotation program then calculates the direction and amount of any turning, as well as total activity, and this data is recorded on diskfile for later analysis. Because the system can operate around the clock unattended, data can be collected over long experimental periods, and detailed circadian analysis of rotation and total activity can be made. The design is also applicable to smaller animals, including rodents, and has many advantages over previously available rotometer designs.
由于已经发现多巴胺能黑质纹状体系统的单侧损伤会在灵长类动物以及啮齿动物中诱发旋转行为，我们设计并构建了一种适用于非人类灵长类动物的旋转监测装置。动物通过系绳连接到一个连续旋转电位计，该电位计提供与位置相关的输出电压。该电压通过模数转换器连接到一台IBM个人计算机。旋转程序随后计算任何转动的方向和幅度以及总活动量，并且这些数据被记录在磁盘文件中以供后续分析。由于该系统可以无人值守地全天候运行，因此可以在较长的实验期间收集数据，并且可以对旋转和总活动量进行详细的昼夜节律分析。该设计也适用于包括啮齿动物在内的较小动物，并且相对于以前可用的旋转计设计具有许多优点。
