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基于代谢组学的超高效液相色谱-高分辨质谱联用技术对藏红花不同部位的鉴别。

Discrimination of Different Parts of Saffron by Metabolomic-Based Ultra-Performance Liquid Chromatography Coupled with High-Definition Mass Spectrometry.

机构信息

Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Henan Provincial Engineering Technology Research Center of Economic Crop Seedling, Zhengzhou, 450008, P. R. China.

College of Animal Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, P. R. China.

出版信息

Chem Biodivers. 2019 Oct;16(10):e1900363. doi: 10.1002/cbdv.201900363. Epub 2019 Sep 4.

Abstract

In this study, the metabolite profiling of three different parts of Crocus sativus L. was measured by using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTof-MS/MS). Multivariate statistical analysis was used to distinguish among the samples from different parts. A total of 54 compounds were identified in tepals, stigmas and stamens by UPLC-QTof-MS/MS. The results stated that chemical characteristics of saffron were obviously diverse in terms of the parts of flower. Through analysis, coniferin and crocin-2 were special components in stigmas when compared to tepals and stamens. The content of flavonoids was high in tepals when compared with the stigmas. The tepal of saffron may processed as a source of flavonoids in the future. The research provided the basis for the theory that only the stigma can be used as medicine.

摘要

本研究采用超高效液相色谱-四极杆飞行时间质谱联用技术(UPLC-QTof-MS/MS)对藏红花的三个不同部位进行了代谢组学分析。通过多元统计分析对不同部位的样品进行了区分。通过 UPLC-QTof-MS/MS 在花瓣、柱头和雄蕊中鉴定出 54 种化合物。结果表明,藏红花的化学成分在花的不同部位明显不同。通过分析,与花瓣和雄蕊相比,松柏醇和藏红花苷-2是柱头的特有成分。花瓣中的类黄酮含量高于柱头。藏红花的花瓣未来可能会被加工成黄酮类化合物的来源。该研究为只有柱头可入药的理论提供了依据。

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