Majewska M D, Mienville J M, Vicini S
FIDIA-Georgetown University for the Neurosciences, Georgetown University Medical School, Washington, DC 20007.
Neurosci Lett. 1988 Aug 1;90(3):279-84. doi: 10.1016/0304-3940(88)90202-9.
Our earlier biochemical studies suggested that the neurosteroid pregnenolone sulfate (PS) may reduce gamma-aminobutyric acid (GABA) action at the Cl- channel associated with GABAA receptors. In the present electrophysiological study the interaction of PS with the GABAA receptor was tested, using whole-cell voltage-clamp recordings from isolated cerebral cortical neurons of neonatal rats. At micromolar concentrations PS reversibly inhibited GABA-induced current, behaving as an allosteric receptor antagonist.
我们早期的生化研究表明，神经甾体硫酸孕烯醇酮（PS）可能会降低γ-氨基丁酸（GABA）在与GABAA受体相关的氯离子通道上的作用。在目前的电生理研究中，我们利用新生大鼠离体大脑皮层神经元的全细胞膜片钳记录，测试了PS与GABAA受体的相互作用。在微摩尔浓度下，PS可逆地抑制GABA诱导的电流，表现为变构受体拮抗剂。
