College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, 443002, China.
Tianjin Key Laboratory of Biomedical Materials, Biomedical Barriers Research Center, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking, Union Medical College, Tianjin, 300192, China.
Macromol Biosci. 2019 Sep;19(9):e1900105. doi: 10.1002/mabi.201900105. Epub 2019 Aug 6.
Polymeric microcapsules have begun to attract significant interest in biomedical fields. As the interactions between cells and materials are influenced by both cell type and elasticity, silk-based microcapsules are synthesized with desirable mechanical features using layer-by-layer assembly and then the uptake of these microcapsules by BeWo b30 placental cells is investigated. Cellular uptake is enhanced with increasing of elastic modulus of the silk-based microcapsules. More importantly, the distinct microvilli of these cells behaves in a diverse manner when exposed to microcapsules with different mechanical features, including grabbing (rigidity) or random touching (soft) behavior; these factors affect the final uptake. Inspired by oocyte pickup, the grabbing behavior of the microvilli may provide valuable information with which to elucidate the specific characteristics of uptake between cells and man-made particles, particularly in the reproductive system.
高分子微胶囊已开始在生物医学领域引起广泛关注。由于细胞与材料的相互作用受到细胞类型和弹性的影响，因此使用层层组装的方法合成具有理想机械性能的丝素基微胶囊，然后研究这些微胶囊被 BeWo b30 胎盘细胞的摄取情况。随着丝素基微胶囊弹性模量的增加，细胞摄取量也随之增加。更重要的是，当这些细胞接触到具有不同机械性能的微胶囊时，其明显的微绒毛会表现出不同的行为，包括抓取（刚性）或随机接触（柔软）行为；这些因素会影响最终的摄取量。受卵母细胞采集的启发，微绒毛的抓取行为可能提供有价值的信息，有助于阐明细胞与人工颗粒之间摄取的特定特征，特别是在生殖系统中。
