Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
J Appl Microbiol. 2019 Nov;127(5):1339-1348. doi: 10.1111/jam.14408. Epub 2019 Sep 1.
This study was conducted to evaluate the chemical composition and in vitro gas production (GP) and fermentation parameters of Quercus infectoria and Quercus libani leaves following treatment with the Klebsiella pneumoniae, a tannin-degrading bacterium.
This isolate was isolated on medium containing tannic acid as the sole source of carbon and energy, and identified based on 16S rRNA sequencing analysis. In both oak leaf species (i.e. Q. infectoria and Q. libani), inoculation with Klebsiella pneumoniae significantly increased (P < 0·05) dry matter (DM) loss. For Q. libani, crude protein content was increased (P = 0·02) by bacterial treatment vs. control. In both oak leaves, total phenolic content and total tannins were decreased (P < 0·05) as a consequence of bacterial treatment. However, bacterial processing didn't changed (P > 0·05) organic matter (OM), neutral detergent fibre, acid detergent fibre or acid detergent lignin content of treated leaves. In both oak leaves the measuring parameters including GP volume, in vitro digestibility of DM and OM, estimated metabolizable energy, total volatile fatty acids, acetate, ammonia nitrogen concentration, total protozoal population and the subfamily Isotricha in treatments were higher (P < 0·05) than control.
It can be concluded that biological treatment of Q. infectoria and Q. libani leaves with K. pneumoniae represents a useful approach to decrease their phenolic compound content and improve their nutritive value as ruminant feed.
This study demonstrated that biologically processing of tannin-containing by-products with K. pneumoniae could increase their nutritive value as ruminant feeds and increase animal productivity.
本研究旨在评估经产单宁酸降解菌 Klebsiella pneumoniae 处理后的栓皮栎和黎巴嫩柽柳叶的化学成分、体外产气量（GP）和发酵参数。
该分离株是在含有单宁酸作为唯一碳源和能源的培养基上分离得到的，并通过 16S rRNA 测序分析进行了鉴定。在两种栎树叶（即栓皮栎和黎巴嫩柽柳）中，接种 Klebsiella pneumoniae 可显著增加（P<0.05）干物质（DM）损失。与对照相比，Klebsiella pneumoniae 处理使黎巴嫩柽柳的粗蛋白含量增加（P=0.02）。在两种栎树叶中，由于细菌处理，总酚含量和总单宁含量均降低（P<0.05）。然而，细菌处理并没有改变（P>0.05）处理叶的有机物（OM）、中性洗涤剂纤维、酸性洗涤剂纤维或酸性洗涤剂木质素含量。在两种栎树叶中，处理组的产气量（GP）体积、DM 和 OM 的体外消化率、估计可代谢能、总挥发性脂肪酸、乙酸、氨氮浓度、总原生动物数量和亚科 Isotricha 等测量参数均高于对照组（P<0.05）。
可以得出结论，用 Klebsiella pneumoniae 对栓皮栎和黎巴嫩柽柳叶片进行生物处理可以降低其酚类化合物含量，提高其作为反刍动物饲料的营养价值。
本研究表明，用 Klebsiella pneumoniae 对含单宁的副产品进行生物处理可以提高其作为反刍动物饲料的营养价值，提高动物的生产力。
