Zhenjiang College, Zhenjiang 212028, China.
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
J Invertebr Pathol. 2019 Sep;166:107227. doi: 10.1016/j.jip.2019.107227. Epub 2019 Aug 3.
Beauveria bassiana, a pathogen of the economically important silkworm (Bombyx mori), causes serious losses in the sericulture industry; however, the mechanisms underlying B. bassiana infection and the silkworm response are not fully understood. To obtain new insights into the interaction between B. bassiana and its host, hemolymph samples from fifth instar silkworm larvae infected with B. bassiana were analyzed at 36-h post-inoculation using a label-free LC-MS/MS proteomic technique. In total, 671 proteins were identified in the hemolymph, including 87 differentially expressed proteins, 42 up-regulated and 45 down-regulated in infected larvae. Six were detected only in infected larvae, and five were detected only in uninfected larvae. Based on GO annotations, 48 of the differentially expressed proteins were involved in molecular functions, 42 were involved in biological processes, and 39 were involved in cell components. A KEGG pathway analysis indicated that these differentially expressed proteins participate in 85 signal transduction pathways, including the amoebiasis, MAPK signaling, Hippo signaling, Toll and Imd signaling, and lysosome pathways. The silkworm hemolymph is the main site for B. bassiana replication. We identified differentially expressed proteins involved in the regulation of the host response to B. bassiana infection, providing important experimental data for the identification of key factors contributing to the interaction between the pathogenic fungus and its host.
球孢白僵菌是一种对经济上重要的家蚕（Bombyx mori）具有致病性的病原体，它会给养蚕业造成严重损失；然而，球孢白僵菌感染和家蚕反应的机制尚未完全了解。为了深入了解球孢白僵菌与其宿主之间的相互作用，我们使用无标记 LC-MS/MS 蛋白质组学技术分析了感染球孢白僵菌的五龄家蚕幼虫血液样本，在接种后 36 小时进行分析。在血液中鉴定出了 671 种蛋白质，包括 87 种差异表达蛋白，其中 42 种在感染幼虫中上调，45 种下调。在感染幼虫中检测到 6 种特异性蛋白，在未感染幼虫中检测到 5 种特异性蛋白。根据 GO 注释，48 种差异表达蛋白参与分子功能，42 种参与生物过程，39 种参与细胞成分。KEGG 通路分析表明，这些差异表达蛋白参与了 85 种信号转导途径，包括变形虫病、MAPK 信号、Hippo 信号、Toll 和 Imd 信号以及溶酶体途径。家蚕血液是球孢白僵菌复制的主要场所。我们鉴定了参与宿主对球孢白僵菌感染反应调控的差异表达蛋白，为鉴定与致病真菌及其宿主相互作用相关的关键因素提供了重要的实验数据。
