Zhenjiang College, Zhenjiang, 212028, China.
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, China.
Mol Immunol. 2021 Jul;135:204-216. doi: 10.1016/j.molimm.2021.04.018. Epub 2021 Apr 28.
Beauveria bassiana is a harmful pathogen to the economically important insect silkworm, always causes serious disease to the silkworm, which results in great losses to the sericulture industry. In order to explore the silkworm (Bombyx mori) response to B. bassiana infection, differential proteomes of the silkworm responsive to B. bassiana infection were identified with isobaric tags for relative and absolute quantitation (iTRAQ) at the different stage of the 3rd instar silkworm larvae. Among the 5040 proteins identified with confidence level of ≥95 %, total 937 proteins were differentially expressed, of which 488 proteins were up-regulated and 449 proteins were down-regulated. 23, 15, 250, 649 differentially expressed proteins (DEPs) were reliably quantified by iTRAQ analysis in the B. bassiana infected larvae at 18, 24, 36, 48 h post infection (hpi) respectively. Based on GO annotations, 6, 4, 128, 316 DEPs were involved in biological processes, 12, 5, 143, 376 DEPs were involved in molecular functions, and 6, 3, 108, 256 DEPs were involved in cell components at 18, 24, 36, 48 hpi respectively. KEGG pathway analysis displayed that 18, 12, 210, 548 DEPs separately participated in 63, 35, 201, 264 signal transduction pathways at different time of infection, and moreover a higher proportion of DEPs involved in metabolic pathways. The cluster analysis on the DEPs of different infection stages distinguished a co-regulated DEP, lysozyme precursor, which was up-regulated at both the mRNA level and the protein level, indicating that the lysozyme protein kept playing an important role in defending the silkworm against B. bassiana infection. This was the first report using an iTRAQ approach to analyze proteomes of the whole silkworm against B. bassiana infection, which contributes to better understanding the defense mechanisms of silkworm to B. bassiana infection and provides important experimental data for the identification of key factors involved in the interaction between the pathogenic fungus and its host.
球孢白僵菌是一种对经济昆虫家蚕有害的病原体，它总是导致家蚕严重发病，给蚕业造成巨大损失。为了探索家蚕（Bombyx mori）对球孢白僵菌感染的反应，采用同位素标记相对和绝对定量技术（iTRAQ）在 3 龄幼虫的不同阶段鉴定了家蚕对球孢白僵菌感染的差异蛋白质组。在置信水平≥95%的 5040 种蛋白质中，共鉴定出 937 种差异表达蛋白，其中 488 种蛋白上调，449 种蛋白下调。在感染后 18、24、36、48 小时，iTRAQ 分析可靠地定量了 23、15、250、649 个差异表达蛋白（DEPs）。基于 GO 注释，在 18、24、36、48 小时时，6、4、128、316 个 DEPs 参与了生物过程，12、5、143、376 个 DEPs 参与了分子功能，6、3、108、256 个 DEPs 参与了细胞成分。KEGG 途径分析显示，在不同感染时间，18、12、210、548 个 DEPs 分别参与了 63、35、201、264 个信号转导途径，而且更多的 DEPs 参与了代谢途径。对不同感染阶段 DEPs 的聚类分析区分了一个共同调控的 DEP，即溶菌酶前体，它在 mRNA 水平和蛋白水平都上调，表明溶菌酶蛋白在家蚕抵抗球孢白僵菌感染中一直发挥着重要作用。这是首次采用 iTRAQ 方法分析家蚕对球孢白僵菌感染的全虫蛋白质组，有助于更好地理解家蚕对球孢白僵菌感染的防御机制，并为鉴定与病原菌及其宿主相互作用相关的关键因素提供了重要的实验数据。
