Hard Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran.
Hard Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran.
J Mech Behav Biomed Mater. 2019 Dec;100:103378. doi: 10.1016/j.jmbbm.2019.103378. Epub 2019 Aug 1.
Over the past decades, the preparation of antibacterial restorative dental adhesives has obtained increasing attention in order to prevent secondary caries. In the present study, a novel essential oil-based antibacterial resin adhesive was prepared and evaluated for dental applications. In this regards, thymol, which is a major phenolic component of thyme essential oil, was incorporated into methacrylate resin matrix and its effect on the physico-mechanical and biological properties of the experimental bonding agent was investigated.
Mechanical properties were evaluated via measuring flexural strength, flexural modulus and fracture toughness. Degree of conversion (DC%) of monomers was measured using FTIR spectroscopy. Viscoelastic properties of the samples were also determined by dynamic mechanical thermal analysis (DMTA). The bactericidal activity of composite specimens against Streptococcus mutans (ATCC 35668) was determined based on ASTM E 2180-07.MTT assay was performed to investigate the cytocompatibility of samples. Furthermore, the bonding strength of the adhesives was evaluated through microshear bond test on the caries-free extracted human premolar teeth and the mode of failure was investigated by scanning electron microscopy.
Thymol-doped resin adhesive exhibited comparable degree of conversion to the control resin adhesive. The plasticizing behavior of thymol slightly decreased the flexural modulus and glass transition temperature of the thymol containing specimens, even though; it caused significant increases in fracture toughness of adhesive. The results represented appropriate antibacterial activity as well as suitable cytocompatibility. Furthermore, the thymol-doped resin adhesive showed comparable adhesive strength to the control.
The thymol is extremely compatible with the methacrylate resin restorative system and completely fulfills all requirements of a good bactericidal component in construction of an ideal enamel bonding system.
在过去的几十年中，为了预防继发龋，人们越来越关注制备抗菌修复牙科胶粘剂。在本研究中，制备了一种新型的基于精油的抗菌树脂胶粘剂，并对其牙科应用进行了评估。在这方面，百里香酚是百里香油的主要酚类成分之一，被掺入甲基丙烯酸酯树脂基质中，并研究了其对实验性粘接剂的物理力学和生物学性能的影响。
通过测量弯曲强度、弯曲模量和断裂韧性来评估机械性能。使用傅里叶变换红外光谱法测量单体的转化率（DC%）。通过动态力学热分析（DMTA）还确定了样品的粘弹性。根据 ASTM E 2180-07 标准，测定复合试件对变形链球菌（ATCC 35668）的杀菌活性。通过 MTT 测定法研究了样品的细胞相容性。此外，通过在无龋人前磨牙上进行微剪切结合试验评估了胶粘剂的结合强度，并通过扫描电子显微镜观察了失效模式。
百里香酚掺杂树脂胶粘剂的转化率与对照树脂胶粘剂相当。百里香的塑化作用略微降低了含百里香酚的试样的弯曲模量和玻璃化转变温度，尽管它显著提高了胶粘剂的断裂韧性。结果表明其具有适当的抗菌活性和适宜的细胞相容性。此外，百里香酚掺杂树脂胶粘剂的结合强度与对照胶粘剂相当。
百里香与甲基丙烯酸酯树脂修复系统非常兼容，完全满足构建理想釉质粘接系统所需的良好杀菌成分的所有要求。
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