Witt S, Dietz H, Ziegler B, Keilacker H, Ziegler M
Zentralinstitut für Diabetes Gerhardt Katsch, Karlsburg, DDR.
Acta Histochem Suppl. 1988;35:217-23.
Murine monoclonal antibodies against glucagon and insulin were generated by somatic cell hybridization and partially characterized. The monoclonal glucagon antibody K79bB10 exhibited no cross-reaction with gut glucagon. This antibody and the insulin antibody K36aC10 were found of very high concentration in ascites. An ascites dilution of 1:5,000 was used for immunohistochemical staining of insulin and glucagon on Bouin-fixed pancreatic tissue sections. By indirect immunofluorescence technique we could demonstrate a reduction of pancreatic insulin in Lewis rats after treatment with complete Freund's adjuvant. The glucagon staining was not altered. The results were confirmed by radioimmunoassay analysis of pancreatic insulin and glucagon content.
通过体细胞杂交产生了抗胰高血糖素和胰岛素的鼠单克隆抗体，并对其进行了部分特性鉴定。单克隆胰高血糖素抗体K79bB10与肠胰高血糖素无交叉反应。发现该抗体和胰岛素抗体K36aC10在腹水中浓度非常高。腹水1:5000的稀释液用于对经Bouin固定的胰腺组织切片上的胰岛素和胰高血糖素进行免疫组织化学染色。通过间接免疫荧光技术，我们可以证明用完全弗氏佐剂处理后的Lewis大鼠胰腺胰岛素减少。胰高血糖素染色未改变。胰腺胰岛素和胰高血糖素含量的放射免疫分析证实了这些结果。
