儿童肥胖中氨基酸、脂质和葡萄糖代谢特征之间的关联。
Associations among amino acid, lipid, and glucose metabolic profiles in childhood obesity.
机构信息
Department of Pediatrics, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto Prefecture, 860-8556, Japan.
Department of Central Radiology, Kumamoto University Hospital, Kumamoto University, Kumamoto City, Kumamoto, Japan.
出版信息
BMC Pediatr. 2019 Aug 6;19(1):273. doi: 10.1186/s12887-019-1647-8.
BACKGROUND
Plasma-free amino acid profiles have been reported to correlate with obesity and glucose metabolism, and have been studied as potentially useful biomarkers of lifestyle-related diseases affecting metabolism in adulthood. However, knowledge of these relationships is lacking in children, despite the growing public health problem posed by childhood obesity. The aim of this study was to assess whether plasma-free amino acid profiles can serve as useful biomarkers of lifestyle-related diseases in children with obesity.
METHODS
This retrospective study used the medical records of 26 patients (15 male, 11 female) aged 9 or 10 years presenting with moderate to severe obesity and hyperlipidemia between April 2015 and March 2017. A degree of obesity of 30% or more was defined as moderate or severe. Amino acid levels were compared between obese children with and without impaired glucose tolerance using a t-test or Mann-Whitney U test. In addition, the influence of factors such as intima media thickness, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, amino acids, and homeostasis model assessment-insulin resistance (HOMA-IR) were analyzed pairwise using Pearson's correlation or Spearman's rank correlation.
RESULTS
HOMA-IR was positively correlated with valine, leucine (Leu), isoleucine, phenylalanine, tryptophan, methionine, threonine, lysine, alanine, tyrosine, glutamate (Glu), proline, arginine, ornithine, total free amino acids (all P < 0.01), and aspartate (P = 0.010). Moreover, blood uric acid levels were positively correlated with Leu (P = 0.005) and Glu (P = 0.019), and negatively correlated with serine, glycine, and asparagine (P = 0.007, P = 0.003, and P = 0.013, respectively).
CONCLUSIONS
Amino acid profile reflects impaired glucose tolerance and hyperuricemia at an early stage of obesity. It is therefore a useful marker to inform early intervention in children with obesity, as in adults.
背景
血浆游离氨基酸谱与肥胖和葡萄糖代谢相关，并且已被研究作为与生活方式相关的影响成年期代谢的疾病的潜在有用的生物标志物。然而，尽管儿童期肥胖带来的公共健康问题日益严重，但人们对这些关系的认识在儿童中却很缺乏。本研究旨在评估血浆游离氨基酸谱是否可以作为肥胖儿童生活方式相关疾病的有用生物标志物。
方法
本回顾性研究使用了 2015 年 4 月至 2017 年 3 月期间患有中度至重度肥胖症和高血脂症的 26 名（男 15 名，女 11 名）9 或 10 岁儿童的病历。肥胖程度超过 30%定义为中度或重度肥胖。使用 t 检验或曼-惠特尼 U 检验比较有和无葡萄糖耐量受损的肥胖儿童之间的氨基酸水平。此外，使用皮尔逊相关或斯皮尔曼等级相关分析，对诸如内中膜厚度、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、氨基酸和稳态模型评估胰岛素抵抗（HOMA-IR）等因素的影响进行两两分析。
结果
HOMA-IR 与缬氨酸、亮氨酸（Leu）、异亮氨酸、苯丙氨酸、色氨酸、蛋氨酸、苏氨酸、赖氨酸、丙氨酸、酪氨酸、谷氨酸（Glu）、脯氨酸、精氨酸、鸟氨酸、总游离氨基酸（均 P < 0.01）和天冬氨酸（P = 0.010）呈正相关。此外，血尿酸水平与 Leu（P = 0.005）和 Glu（P = 0.019）呈正相关，与丝氨酸、甘氨酸和天冬酰胺呈负相关（P = 0.007、P = 0.003 和 P = 0.013）。
结论
氨基酸谱反映了肥胖早期的葡萄糖耐量受损和高尿酸血症。因此，它是一种有用的标志物，可以提示肥胖儿童早期干预，就像在成人中一样。
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