一名难治性贫血患者中粒细胞-巨噬细胞和红系谱系克隆性受累的细胞遗传学证据。

Cytogenetic evidence for a clonal involvement of granulocyte-macrophage and erythroid lineages in a patient with refractory anaemia.

作者信息

Okuda T, Yokota S, Maekawa T, Sonoda Y, Taniwaki M, Misawa S, Takino T, Abe T

机构信息

Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.

出版信息

Acta Haematol. 1988;80(2):110-5. doi: 10.1159/000205614.

DOI:10.1159/000205614

PMID:3138872

Abstract

To investigate the clonal origin of refractory anaemia, we carried out cytogenetic studies on single haematopoietic colonies derived from granulocyte-macrophage precursors (CFU-GM) and erythroid precursors (BFU-E). Marrow cells from a patient with refractory anaemia revealed the coexistence of a normal and an abnormal karyotype; 46,XY/45,XY,-15,-18,+der(15q18q). Cytogenetic studies on CFU-GM- and BFU-E-derived colonies obtained from the bone marrow showed the presence of the same karyotypic abnormality carrying the der(15q18q). Colonies carrying a normal diploid karyotype were also detected in the same culture dish. These results indicate that the clone with the der(15q18q) chromosome abnormality arises in a stem cell which can differentiate to at least both granulocyte-macrophage and erythroid lineages.

摘要

为了研究难治性贫血的克隆起源，我们对源自粒细胞-巨噬细胞前体（CFU-GM）和红系前体（BFU-E）的单个造血集落进行了细胞遗传学研究。一名难治性贫血患者的骨髓细胞显示正常核型和异常核型共存；46,XY/45,XY,-15,-18,+der(15q18q)。对从骨髓获得的CFU-GM和BFU-E来源的集落进行的细胞遗传学研究显示存在携带der(15q18q)的相同核型异常。在同一培养皿中也检测到携带正常二倍体核型的集落。这些结果表明，具有der(15q18q)染色体异常的克隆起源于一个干细胞，该干细胞至少可以分化为粒细胞-巨噬细胞和红系谱系。