Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.
Guangdong Shuanglin Bio-Pharmacy Co, Ltd, Zhanjiang, China.
J Med Virol. 2019 Dec;91(12):2059-2065. doi: 10.1002/jmv.25538. Epub 2019 Aug 31.
Zika virus (ZIKV) is an emerging arbovirus with increasing prevalence in recent years. To reduce the risk of ZIKV transmission by transfusion, some mitigation strategies were recommended based on pathogen reduction technologies for blood products. In this study, we aimed to study the efficacy of several common pathogen reduction methods in the inactivation of ZIKV. The fresh frozen plasma and derivatives were spiked with a high titer of ZIKV or Sindbis virus (SINV). Viral titers and ZIKV RNA were measured before and after the inactivation treatment by methylene blue (MB), solvent/detergent (S/D), pasteurization, and low pH. The mean ZIKV infectivity titers in plasma and derivatives were 7.08 ± 0.14, 5.17 ± 0.14, 7.08 ± 0.14, and 5.80 ± 0.14 log TCID /mL, respectively before MB, S/D, pasteurization, and low pH inactivation. We found no detectable ZIKV RNA after five successive passages of inoculation on host cells, indicating there is no infectivity after inactivation. Similar inactivation results were observed for SINV. In conclusion, we achieved robust ZIKV inactivation through the four inactivation procedures in several blood products. These findings suggest that the pathogen reduction technologies commonly applied in plasma and derivatives have the capacity to mitigate the risk of ZIKV transmission by transfusion.
Zika 病毒（ZIKV）是一种新兴的虫媒病毒，近年来发病率不断上升。为了降低输血传播 ZIKV 的风险，根据血液制品的病原体减少技术推荐了一些缓解策略。在这项研究中，我们旨在研究几种常见的病原体减少方法对 ZIKV 灭活的效果。新鲜冷冻血浆及其衍生物中加入高滴度的 ZIKV 或辛德毕斯病毒（SINV）。在使用亚甲蓝（MB）、溶剂/去污剂（S/D）、巴氏消毒和低 pH 值进行灭活处理前后，通过测量病毒滴度和 ZIKV RNA 来评估病毒的感染力。在 MB、S/D、巴氏消毒和低 pH 值灭活之前，血浆及其衍生物中的 ZIKV 感染性滴度分别为 7.08±0.14、5.17±0.14、7.08±0.14 和 5.80±0.14 log TCID /mL。我们发现，经过连续五次接种宿主细胞后，没有检测到 ZIKV RNA，这表明在灭活后没有感染性。SINV 也观察到了类似的灭活结果。总之，我们通过在几种血液制品中进行的四种灭活程序实现了强大的 ZIKV 灭活效果。这些发现表明，在血浆及其衍生物中广泛应用的病原体减少技术有能力降低输血传播 ZIKV 的风险。
