Department of Orthopaedics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):54-59. doi: 10.26355/eurrev_201908_18628.
To explore the clinical correlation between the hsa-miR-122-3p expression in bone marrow mesenchymal stem cells (BMSCs) and steroid-induced necrosis of femoral head (SONFH).
A total of 62 SONFH patients were selected as the experimental group, while another 72 patients with femoral neck fracture (FNF) were selected as the control group. The bone marrow was obtained from patients during operation and used to culture the BMSCs. The expression of hsa-miR-122-3p in BMSCs was detected via real-time quantitative polymerase chain reaction (qPCR) in both groups. The patients in experimental group were further divided into Ficat stage III group and stage IV group according to the Ficat stage, and the expression of hsa-miR-122-3p in BMSCs was also detected via qPCR in the two groups.
The expression level of hsa-miR-122-3p in SONFH group was significantly lower than that in FNF group, and the difference was statistically significant (p<0.05). The expression level of hsa-miR-122-3p in Ficat stage IV group was significantly lower than that in stage III group (p<0.05).
We demonstrated that the expression of hsa-miR-122-3p in BMSCs declined in SONFH group, indicating that hsa-miR-122-3p may be involved in the regulation of the pathological process of SONFH, and the expression level of hsa-miR-122-3p in BMSCs may be correlated with the progression of SONFH.
探讨骨髓间充质干细胞（BMSCs）中 hsa-miR-122-3p 的表达与激素性股骨头坏死（SONFH）的临床相关性。
选择 62 例 SONFH 患者作为实验组，另选择 72 例股骨颈骨折（FNF）患者作为对照组。术中获取患者骨髓，用于培养 BMSCs。采用实时定量聚合酶链反应（qPCR）检测两组 BMSCs 中 hsa-miR-122-3p 的表达。根据 Ficat 分期，实验组患者进一步分为 Ficat Ⅲ期和Ⅳ期组，采用 qPCR 检测两组 BMSCs 中 hsa-miR-122-3p 的表达。
SONFH 组 hsa-miR-122-3p 的表达水平明显低于 FNF 组，差异有统计学意义（p<0.05）。Ficat Ⅳ期组 hsa-miR-122-3p 的表达水平明显低于Ⅲ期组（p<0.05）。
我们证实 BMSCs 中 hsa-miR-122-3p 的表达在 SONFH 组下降，表明 hsa-miR-122-3p 可能参与 SONFH 病理过程的调节，BMSCs 中 hsa-miR-122-3p 的表达水平可能与 SONFH 的进展相关。
