Department of Orthopedic Surgery, The Traditional Chinese Medicine Affiliated Hospital of Southwest Medical University, Luzhou, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):60-66. doi: 10.26355/eurrev_201908_18629.
To explore the role of long non-coding ribonucleic acid-anti-differentiation non-coding ribonucleic acid (lncRNA-ANCR) in tibial fracture healing in rabbits by regulating the runt-related transcription factor 2 (RUNX2) expression.
A total of 60 healthy adult rabbits were evenly divided into Control group (n=20), Fracture group (n=20), and Lnc group (n=20). Then, RUNX2 transfection, Real Time Polymerase Chain Reaction (PCR) assay and relevant instruments were carried out and used to determine the differences in dry weight, bone mineral density, bone mechanical strength, and RUNX2 expression in tibiae among three groups of rabbits.
Comparison of the bone mineral density in rabbit tibiae among the three groups showed that the bone mineral density was significantly lower in Fracture group than that in Control group (p<0.05), and it was slightly higher in Lnc group than in Fracture group (p<0.05). The dry weight of the full-length tibiae in Fracture group was significantly decreased compared with that in Control group (p<0.05), and Lnc group had an increased dry weight of tibiae in comparison with Fracture group (p<0.05). The maximum load, flexural strength, elastic stress, elastic strain, elastic modulus, maximum stress, and maximum strain in Fracture group were lower than those in both Control group and Lnc group (p<0.05). Compared with those in Fracture group, the amount of new collagen was overtly increased in Lnc group, and that of mature collagen was decreased (p<0.05). The relative expression level of RUNX2 in tibial bone tissues was evidently lower in Fracture group than that in Control group (p<0.05), and it was markedly higher in Lnc group than that in Fracture group (p<0.05).
Down-regulating lncRNA-ANCR activates and triggers the expression of RUNX2 that facilitates the growth and metabolism of bone tissues to play an important role in the repair of bone tissues and promote the healing of the tibial fracture.
通过调控 runt 相关转录因子 2（RUNX2）的表达，探讨长链非编码 RNA-反分化非编码 RNA（lncRNA-ANCR）在兔胫骨骨折愈合中的作用。
将 60 只健康成年兔平均分为对照组（n=20）、骨折组（n=20）和 Lnc 组（n=20）。然后，对 RUNX2 转染、实时聚合酶链反应（PCR）检测及相关仪器进行检测，以确定三组兔胫骨的干重、骨矿物质密度、骨力学强度和 RUNX2 表达的差异。
三组兔胫骨骨矿物质密度比较，骨折组骨矿物质密度明显低于对照组（p<0.05），Lnc 组略高于骨折组（p<0.05）。与对照组相比，骨折组全长胫骨干重明显降低（p<0.05），Lnc 组胫骨干重增加（p<0.05）。骨折组的最大负荷、弯曲强度、弹性应力、弹性应变、弹性模量、最大应力和最大应变均低于对照组和 Lnc 组（p<0.05）。与骨折组相比，Lnc 组的新生胶原明显增多，成熟胶原明显减少（p<0.05）。骨折组胫骨组织中 RUNX2 的相对表达水平明显低于对照组（p<0.05），Lnc 组明显高于骨折组（p<0.05）。
下调 lncRNA-ANCR 可激活并触发 RUNX2 的表达，促进骨组织的生长和代谢，在骨组织修复中发挥重要作用，促进胫骨骨折愈合。
