Qu C-X, Shi X-C, Zai L-Q, Bi H, Yang Q
Department of Pathology, Shanxi People's Hospital, Taiyuan, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):247-255. doi: 10.26355/eurrev_201908_18654.
To uncover the biological role of long non-coding RNA (lncRNA) CASC19 in the pathogenesis of non-small cell lung carcinoma (NSCLC) and the potential mechanism.
Expression pattern of lncRNA CASC19 in NSCLC tissues and cell lines was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Survival analysis on the correlation between CASC19 level and prognosis of NSCLC patients was conducted by introducing for the Kaplan-Meier estimator. After the transfection of si-CASC19 in A549 and PC9 cells, changes in viability, migratory, and invasive capacities were evaluated. Dual-luciferase reporter gene assay was performed to explore the interaction between microRNA-130b-3p (miRNA-130b-3p) and CASC19/ZEB2. Their interactive effects on the progression of NSCLC were finally investigated through rescue experiments.
LncRNA CASC19 was upregulated in NSCLC tissues and cell lines. NSCLC patients with high expression of CASC19 presented a worse survival. Knockdown of CASC19 attenuated proliferative, migratory, and invasive capacities of A549 and PC9 cells. CASC19 sponged miRNA-130b-3p and negatively regulated its level. ZEB2 was the direct target of miRNA-130b-3p. The knockdown of miRNA-130b-3p reversed the regulatory effects of CASC19 on A549 and PC9 cells.
CASC19 sponges miRNA-130b-3p to regulate ZBR2 as a ceRNA, thus accelerating the progression of NSCLC by regulating proliferative, migratory, and invasive capacities of tumor cells.
揭示长链非编码RNA（lncRNA）CASC19在非小细胞肺癌（NSCLC）发病机制中的生物学作用及潜在机制。
采用定量实时聚合酶链反应（qRT-PCR）检测lncRNA CASC19在NSCLC组织和细胞系中的表达模式。通过引入Kaplan-Meier估计量对CASC19水平与NSCLC患者预后的相关性进行生存分析。在A549和PC9细胞中转染si-CASC19后，评估细胞活力、迁移和侵袭能力的变化。进行双荧光素酶报告基因实验以探究微小RNA-130b-3p（miRNA-130b-3p）与CASC19/ZEB2之间的相互作用。最后通过挽救实验研究它们对NSCLC进展的交互作用。
lncRNA CASC19在NSCLC组织和细胞系中上调。CASC19高表达的NSCLC患者生存较差。敲低CASC19可减弱A549和PC9细胞的增殖、迁移和侵袭能力。CASC19吸附miRNA-130b-3p并负向调节其水平。ZEB2是miRNA-130b-3p的直接靶标。敲低miRNA-130b-3p可逆转CASC19对A549和PC9细胞的调节作用。
CASC19作为竞争性内源性RNA吸附miRNA-130b-3p以调节ZBR2，从而通过调节肿瘤细胞的增殖、迁移和侵袭能力加速NSCLC的进展。
