Department of Chemistry, National Tsing Hua University, Hsinchu, 30013, Taiwan.
Department of Chemistry&Biochemistry, University of California, Santa Barbara, CA, 93106-9510, USA.
Chem Asian J. 2019 Nov 18;14(22):3981-3991. doi: 10.1002/asia.201900855. Epub 2019 Aug 23.
Spin-label electron spin resonance (ESR) has emerged as a powerful tool to characterize protein dynamics. One recent advance is the development of ESR for resolving dynamical components that occur or coexist during a biological process. It has been applied to study the complex structural and dynamical aspects of membranes and proteins, such as conformational changes in protein during translocation from cytosol to membrane, conformational exchange between equilibria in response to protein-protein and protein-ligand interactions in either soluble or membrane environments, protein oligomerization, and temperature- or hydration-dependent protein dynamics. As these topics are challenging but urgent for understanding the function of a protein on the molecular level, the newly developed ESR methods to capture individual dynamical components, even in low-populated states, have become a great complement to other existing biophysical tools.
自旋标记电子自旋共振（ESR）已成为一种强大的工具，用于研究蛋白质动力学。最近的一项进展是开发 ESR 技术，以解析在生物过程中发生或共存的动力学成分。该技术已被应用于研究膜和蛋白质的复杂结构和动力学方面，例如蛋白质在从细胞质到膜的转运过程中的构象变化、在可溶性或膜环境中蛋白质-蛋白质和蛋白质-配体相互作用响应下平衡之间的构象交换、蛋白质寡聚化以及温度或水合依赖性蛋白质动力学。由于这些主题对于理解蛋白质在分子水平上的功能具有挑战性但又十分紧迫，因此开发新的 ESR 方法来捕获单个动力学成分，即使在低 populate 状态下，也成为其他现有生物物理工具的很好补充。
