Banga H S, Walker R K, Winberry L K, Rittenhouse S E
Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
Biochem J. 1988 May 15;252(1):297-300. doi: 10.1042/bj2520297.
Thrombin stimulates phospholipase C and inhibits adenylate cyclase in human platelets. We have studied the effect of purified S1 monomer, the ADP-ribosylating subunit of pertussis toxin, on these receptor-coupled G-protein-dependent activities. ADP-ribosylation of a 41 kDa protein is associated with a marked decrease in the ability of thrombin to inhibit cyclic AMP formation, but has little effect on phospholipase C. Therefore adenylate cyclase and phospholipase C appear to be modulated by different G-proteins.
凝血酶可刺激人血小板中的磷脂酶C并抑制腺苷酸环化酶。我们研究了纯化的S1单体（百日咳毒素的ADP-核糖基化亚基）对这些受体偶联的G蛋白依赖性活性的影响。41 kDa蛋白的ADP-核糖基化与凝血酶抑制环磷酸腺苷形成能力的显著降低有关，但对磷脂酶C影响不大。因此，腺苷酸环化酶和磷脂酶C似乎受不同G蛋白的调节。
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