Centre for Research in Biosciences, Faculty of Health and Applied Sciences, University of the West of England, Frenchay Campus, Coldharbour Lane, Bristol BS16 1QY, UK.
Biosensors (Basel). 2019 Aug 6;9(3):98. doi: 10.3390/bios9030098.
An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its electrochemically active thiolate anion species. It was shown that an electrocatalytic oxidation reaction occurred with the screen-printed carbon electrode containing the mediator cobalt phthalocyanine (CoPC-SPCE). This had the advantage of producing an analytical response current at an operating potential of 0 V vs. Ag/AgCl compared to +0.34 V obtained with plain SPCEs. This resulted in improved selectivity and limit of detection. Detailed studies on the underlying mechanism occurring with the sensor are reported in this paper. A linear response was obtained between 0.1 and 20 µg mL, which was suitable for the quantification of the vitamin in two commercial products containing vitamin B1. The mean recovery for a multivitamin tablet with a declared content of 5 mg was 101% (coefficient of variation (CV) of 9.6%). A multivitamin drink, which had a much lower concentration of vitamin B1 (0.22 mg/100 mL), gave a mean recovery of 93.3% (CV 7.2%). These results indicate that our sensor holds promise for quality control of food supplements and other food types.
一种电化学催化的丝网印刷传感器已被首次用于食品补充剂中对重要生物分子维生素 B1（硫胺素）的测量。在碱性条件下，维生素被转化为其具有电化学活性的硫醇盐阴离子物种。研究表明，在含有介体钴酞菁（CoPC-SPCE）的丝网印刷碳电极上发生了电催化氧化反应。与使用普通 SPCE 获得的 +0.34 V 相比，该反应在 0 V vs. Ag/AgCl 的工作电位下产生了分析响应电流，这具有提高选择性和检测限的优点。本文详细报道了传感器中发生的潜在机制的研究。在 0.1 至 20 µg mL 之间获得了线性响应，适用于两种含有维生素 B1 的商业产品中维生素的定量。对于一种宣称含有 5 mg 维生素的多种维生素片剂，平均回收率为 101%（变异系数（CV）为 9.6%）。对于维生素 B1 浓度低得多的多种维生素饮料（100 mL 中含 0.22 mg），平均回收率为 93.3%（CV 为 7.2%）。这些结果表明，我们的传感器有望用于食品补充剂和其他食品类型的质量控制。
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