Lgr5肠道干细胞分选及类器官培养。
Lgr5 intestinal stem cell sorting and organoid culture.
作者信息
Liu Ruixue, Li Haifeng, Cai Juan, Wei Qiang, Han Xiaonan
机构信息
Key Laboratory of Human Disease Comparative Medicine The Ministry of Health Institute of Laboratory Animal Science (ILAS) Chinese Academy of Medical Science (CAMS) and Peking Union Medical College (PUMC) Beijing P.R. China.
出版信息
Animal Model Exp Med. 2019 Mar 12;2(2):132-135. doi: 10.1002/ame2.12057. eCollection 2019 Jun.
Intestinal epithelial stem cells (IESCs) are one of the most rapidly self-renewing and proliferating adult stem cells. The IESCs reside at the bottom of intestinal and colonic crypts, giving rise to all intestinal epithelial lineages and maintaining intestinal epithelial replenishment. The technique of three-dimensional culture based upon intestinal stem cell biology has been recently developed to study gastrointestinal development and disease pathogenesis. Here, we summarize the techniques used to isolate Lgr5-positive IESCs to form the enteroids from intestine or colonoids from colon, and present the means to examine these organoid functions. This study will provide a simple and practical way for producing intestinal tissues in the laboratory.
肠道上皮干细胞（IESCs）是自我更新和增殖速度最快的成体干细胞之一。IESCs 位于小肠和结肠隐窝底部，可分化为所有肠道上皮谱系并维持肠道上皮细胞的更新。基于肠道干细胞生物学的三维培养技术最近已被开发出来，用于研究胃肠道发育和疾病发病机制。在这里，我们总结了用于从小肠中分离 Lgr5 阳性 IESCs 以形成肠类器官或从结肠中分离 Lgr5 阳性 IESCs 以形成结肠类器官的技术，并介绍了检测这些类器官功能的方法。本研究将为在实验室中生产肠道组织提供一种简单实用的方法。
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