Department of Radiology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, 20# Section 3 South Renmin Road, Chengdu, China.
Department of Radiology, West China Hospital, Sichuan University, China.
NMR Biomed. 2019 Nov;32(11):e4158. doi: 10.1002/nbm.4158. Epub 2019 Aug 8.
We developed a novel manganese (Mn ) chelate for magnetic resonance imaging (MRI) assessment of myocardial viability in acute and chronic myocardial infarct (MI) models, and compared it with Gadolinium-based delay enhancement MRI (Gd -DEMRI) and histology. MI was induced in 14 rabbits by permanent occlusion of the left circumflex coronary artery. Gd -DEMRI and Mn chelate-based delayed enhancement MRI (Mn chelate-DEMRI) were performed at 7 days (acute MI, n = 8) or 8 weeks (chronic MI, n = 6) after surgery with sequential injection of 0.15 mmol/kg Gd and Mn chelate. The biodistribution of Mn in tissues and blood was measured at 1.5 and 24 h. Blood pressure, heart rate (HR), left ventricular (LV) function, and infarct fraction (IF) were analyzed, and IF was compared with the histology. The Mn chelate group maintained a stable hemodynamic status during experiment. For acute and chronic MI, all rabbits survived without significant differences in HR or LV function before and after injection of Mn chelate or Gd (p > 0.05). Mn chelate mainly accumulated in the kidney, liver, spleen, and heart at 1.5 h, with low tissue uptake and urine residue at 24 h after injection. In the acute MI group, there was no significant difference in IF between Mn chelate-DEMRI and histology (22.92 ± 2.21% vs. 21.79 ± 2.25%, respectively, p = 0.87), while Gd -DEMRI overestimated IF, as compared with histology (24.54 ± 1.73%, p = 0.04). In the chronic MI group, there was no significant difference in IF between the Mn chelate-DEMRI, Gd -DEMRI, and histology (29.50 ± 11.39%, 29.95 ± 9.40%, and 29.00 ± 10.44%, respectively, p > 0.05), and all three were well correlated (r = 0.92-0.96, p < 0.01). We conclude that the use of Mn chelate-DEMRI is reliable for MI visualization and identifies acute MI more accurately than Gd -DEMRI.
我们开发了一种新型的锰（Mn）螯合物，用于磁共振成像（MRI）评估急性和慢性心肌梗死（MI）模型中的心肌活力，并将其与基于钆的延迟增强 MRI（Gd -DEMRI）和组织学进行了比较。通过永久性阻塞左回旋冠状动脉，在 14 只兔子中诱导 MI。在手术后 7 天（急性 MI，n=8）或 8 周（慢性 MI，n=6）时，使用 0.15 mmol/kg Gd 和 Mn 螯合物进行连续注射，进行 Gd -DEMRI 和 Mn 螯合物延迟增强 MRI（Mn chelate-DEMRI）。在 1.5 和 24 小时时测量 Mn 在组织和血液中的生物分布。分析血压、心率（HR）、左心室（LV）功能和梗死分数（IF），并将 IF 与组织学进行比较。Mn 螯合物组在实验过程中保持稳定的血流动力学状态。对于急性和慢性 MI，在注射 Mn 螯合物或 Gd 前后，所有兔子的 HR 或 LV 功能均无明显差异（p>0.05）。Mn 螯合物在 1.5 小时时主要积聚在肾脏、肝脏、脾脏和心脏中，在注射后 24 小时时，组织摄取量低，尿液残留少。在急性 MI 组中，Mn chelate-DEMRI 与组织学之间的 IF 无显著差异（分别为 22.92±2.21%和 21.79±2.25%，p=0.87），而 Gd -DEMRI 与组织学相比高估了 IF（分别为 24.54±1.73%，p=0.04）。在慢性 MI 组中，Mn chelate-DEMRI、Gd -DEMRI 和组织学之间的 IF 无显著差异（分别为 29.50±11.39%、29.95±9.40%和 29.00±10.44%，p>0.05），且三者均呈高度相关（r=0.92-0.96，p<0.01）。我们的结论是，Mn chelate-DEMRI 的使用可靠地用于 MI 可视化，并且比 Gd -DEMRI 更准确地识别急性 MI。
