Centre for Chemistry and Biotechnology, Deakin University, Locked Bag 20000, Geelong, VIC, Australia.
Seafood Unit, The New Zealand Institute for Plant & Food Research Limited, 293-297 Akersten Street, Nelson 7010, New Zealand.
Food Chem. 2019 Dec 15;301:125297. doi: 10.1016/j.foodchem.2019.125297. Epub 2019 Jul 29.
Candida antarctica lipase B-catalysed synthesis of lipophilic esters of polydatin was investigated along with their antioxidant activities. The effects of synthesis parameters such as solvent, substrate molar ratio, enzyme concentration, addition of molecular sieves, reaction temperature and time on the production of ester were studied and optimised. The highest production of esters was obtained with acetone as the reaction solvent. The antioxidant activities of the esters were compared with those of commercial butylated hydroxytoluene (BHT) and α-tocopherol. All polydatin esters inhibited the oxidative destruction of β-carotene more effectively than did BHT and α-tocopherol. Results of thiobarbituric acid tests showed that in bulk fish oil, all esters were more effective than α-tocopherol at 2 mmol/kg concentration but were not as effective as BHT. In fish oil-emulsions, all esters were more effective than both BHT and α-tocopherol at 2 mmol/kg concentration. The synthesized polydatin esters are promising antioxidants for oil/fat-based foods.
南极假丝酵母脂肪酶 B 催化虎杖苷的亲脂性酯的合成及其抗氧化活性研究。考察了溶剂、底物摩尔比、酶浓度、分子筛添加、反应温度和时间等合成参数对酯合成的影响，并对其进行了优化。以丙酮为反应溶剂时，酯的产率最高。将酯的抗氧化活性与商业叔丁基对羟基甲苯（BHT）和α-生育酚进行了比较。所有的虎杖苷酯对β-胡萝卜素的氧化破坏抑制作用均优于 BHT 和α-生育酚。硫代巴比妥酸试验结果表明，在大量鱼油中，所有酯在 2mmol/kg 浓度下均比α-生育酚更有效，但不如 BHT 有效。在鱼油乳液中，所有酯在 2mmol/kg 浓度下均优于 BHT 和α-生育酚。合成的虎杖苷酯是一种有前途的油/脂肪基食品抗氧化剂。
