Yoshikawa K, Aizawa T
Laboratory of Molecular Biology, Psychiatric Research Institute, Tokyo, Japan.
FEBS Lett. 1988 Sep 12;237(1-2):183-6. doi: 10.1016/0014-5793(88)80197-2.
Searching for somatic cells expressing the preproenkephalin (A) gene in the testis, we have isolated Sertoli cells from the testes of 20-day-old rats. Cultured Sertoli cells contained a single species (about 1.5 kb) of preproenkephalin mRNA, and follicle-stimulating hormone (FSH) transiently increased the mRNA abundance to a maximum (about 30 molecules per cell) at 12 h. Various compounds that activate the cyclic AMP system in Sertoli cells similarly increased the abundance of preproenkephalin mRNA. Moreover, FSH increased intracellular Met-enkephalin immunoreactive peptides in Sertoli cells. Thus, the preproenkephalin gene expression in Sertoli cells is positively regulated by FSH through the cyclic AMP system.
为了寻找睾丸中表达前脑啡肽原（A）基因的体细胞，我们从20日龄大鼠的睾丸中分离出了支持细胞。培养的支持细胞含有一种单一类型（约1.5 kb）的前脑啡肽原mRNA，促卵泡激素（FSH）在12小时时可使mRNA丰度短暂增加至最大值（约每个细胞30个分子）。各种能激活支持细胞中环磷酸腺苷系统的化合物同样增加了前脑啡肽原mRNA的丰度。此外，FSH增加了支持细胞内甲硫氨酸脑啡肽免疫反应性肽。因此，支持细胞中的前脑啡肽原基因表达通过环磷酸腺苷系统受到FSH的正向调节。
