白藜芦醇对MDA-MB-231乳腺癌细胞中PI3K-AKT信号通路基因的调控
Resveratrol Regulation of PI3K-AKT Signaling Pathway Genes in MDA-MB-231 Breast Cancer Cells.
作者信息
Rachid Ousama, Alkhalaf Moussa
机构信息
Department of Biochemistry, Faculty of Medicine, Kuwait University, Safat, Kuwait.
Department of Biochemistry, Faculty of Medicine, Kuwait University, Safat, Kuwait
出版信息
Cancer Genomics Proteomics. 2006 Nov-Dec;3(6):383-388. Epub 2006 Nov 1.
BACKGROUND
Resveratrol (RSVL), a natural compound found in grapes and other food products, has been described to exert cancer chemopreventive activities. However, the cellular and molecular basis of its anticancer activity is largely undefined. The aim of the present study was to identify RSVL target genes in the MDA-MB-231 breast cancer cell line using cDNA arrays representing genes of the PI3K/AKT signaling pathway.
MATERIALS AND METHODS
Total RNA from control and RSVL-treated cells was used to synthesize biotinylated cDNA probes. cDNA arrays were hybridized with the probes and signals were detected with a chemiluminescent method. Western blotting analysis was used to validate the arrays' gene expression.
RESULTS
At the cDNA level, 13 genes were altered (at least 2-fold difference) by RSVL treatment. At the protein level, both c-fos and P70S6K were also regulated.
CONCLUSION
Using gene arrays it was shown for the first time that c-fos and p70S6 kinase were regulated by RSVL.
背景
白藜芦醇（RSVL）是一种存在于葡萄及其他食品中的天然化合物，已被描述具有癌症化学预防活性。然而，其抗癌活性的细胞和分子基础在很大程度上尚不清楚。本研究的目的是使用代表PI3K/AKT信号通路基因的cDNA阵列，在MDA-MB-231乳腺癌细胞系中鉴定RSVL的靶基因。
材料与方法
来自对照细胞和经RSVL处理的细胞的总RNA用于合成生物素化的cDNA探针。cDNA阵列与探针杂交，并用化学发光法检测信号。蛋白质印迹分析用于验证阵列的基因表达。
结果
在cDNA水平上，经RSVL处理后有13个基因发生改变（至少2倍差异）。在蛋白质水平上，c-fos和P70S6K也受到调控。
结论
利用基因阵列首次表明c-fos和p70S6激酶受RSVL调控。
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