Spater H W, Novikoff A B, Spater S H, Quintana N
J Histochem Cytochem. 1978 Oct;26(10):809-21. doi: 10.1177/26.10.31396.
A phosphatase, hydrolyzing pyridoxal-5-phosphate (P5P), a physiologically active component of the vitamin B6 complex and an essential co-enzyme in the synthesis of neurotransmitters, has been localized cytochemically in the perikarya of neurons in the peripheral, autonomic and central nervous systems of the rat. Neurons in dorsal root ganglia, sympathetic ganglia and ventral horn of spinal cord were studied by light and electron microscopy, while Purkinje cells, neurons in the dentate nucleus of the cerebellum, thalamus, and hypothalamus were studied by light microscopy only. Optimal conditions for demonstrating this activity in aldehyde-fixed tissue were determined with dorsal root ganglia. At the optimal pH of 5.0, neurons in these ganglia and in all other neurons studied show pyridoxal-5-phosphatase (P5Pase) activity in GERL. Small neurons in dorsal root ganglia also display enzyme activity in the endoplasmic reticulum (ER); activities in GERL and ER are also appreciably high at neutral pH. Small and large neurons in these ganglia, and neurons of sympathetic ganglia, show variable P5Pase activity in the Golgi apparatus. These localizations differ from the usual sites of both acid phosphatase and alkaline phosphatase activities. The P5Pase activity, demonstrated cytochemically, is a new acid hydrolase activity in GERL.
一种可水解磷酸吡哆醛（P5P）的磷酸酶已通过细胞化学方法定位在大鼠外周、自主和中枢神经系统神经元的胞体中。P5P是维生素B6复合物的一种生理活性成分，也是神经递质合成中必不可少的辅酶。通过光学显微镜和电子显微镜研究了背根神经节、交感神经节和脊髓腹角中的神经元，而仅通过光学显微镜研究了浦肯野细胞、小脑齿状核、丘脑和下丘脑的神经元。以背根神经节为材料，确定了在醛固定组织中显示这种活性的最佳条件。在最佳pH值5.0时，这些神经节以及所有其他研究的神经元中的神经元在GERL中显示出磷酸吡哆醛-5-磷酸酶（P5Pase）活性。背根神经节中的小神经元在内质网（ER）中也表现出酶活性；在中性pH值下，GERL和ER中的活性也相当高。这些神经节中的小神经元和大神经元以及交感神经节的神经元在高尔基体中表现出可变的P5Pase活性。这些定位与酸性磷酸酶和碱性磷酸酶活性的常见部位不同。通过细胞化学方法证明的P5Pase活性是GERL中一种新的酸性水解酶活性。
