Takahashi I, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine, Aichi.
J Biochem. 1988 Apr;103(4):641-3. doi: 10.1093/oxfordjournals.jbchem.a122321.
A rapid purification procedure with high recovery of blood coagulation factor XII (Hageman factor, HF) was established. Homogeneous HF was isolated in 6 days on a monoclonal antibody-immunoaffinity column chromatography followed by gel filtration. Approximately 4,300-fold purification of HF was attained with 31% yield on average (n = 4). Using this method, an abnormal HF was purified from plasma of a patient with cross-reacting material (CRM)-positive Hageman trait (factor XIITORONTO). The abnormal HF was found to be a single chain polypeptide with the same molecular weight (80,000) as the normal HF. Both abnormal and normal HF had similar amino acid compositions.
建立了一种快速纯化程序，可实现高回收率的凝血因子 XII（哈格曼因子，HF）。通过单克隆抗体免疫亲和柱色谱法，随后进行凝胶过滤，在 6 天内分离出了均一的 HF。平均回收率为 31%（n = 4），实现了约 4300 倍的 HF 纯化。使用该方法，从一名具有交叉反应物质（CRM）阳性哈格曼特征（因子 XIITORONTO）的患者血浆中纯化出了异常 HF。发现异常 HF 是一种单链多肽，分子量（80,000）与正常 HF 相同。异常 HF 和正常 HF 的氨基酸组成相似。
