Kuo B S, Korner G, Dryjski M, Bjornsson T D
Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
J Cell Physiol. 1988 Oct;137(1):192-8. doi: 10.1002/jcp.1041370124.
The effects of the polyamines putrescine (PUT), spermidine (SPD), and spermidine (SPM) on the secretion of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) were evaluated using cultured bovine aortic endothelial cells. All three polyamines enhanced PA secretion in a time- and dose-dependent manner, with a potency rank order of SPM greater than SPD greater than PUT. The PA stimulation required both RNA and protein synthesis, as evidenced by inhibition of polyamine-induced PA secretion by actinomycin D and cycloheximide. The inhibitors of polyamine biosynthesis methylglyoxal bis-(guanylhydrazone) (MGBG) and dl-(difluoromethyl) ornithine (DFMO) alone did not affect basal or polyamine-induced PA secretion, with the exception that MGBG reduced the effect of PUT. Polyamine-treated cells enhanced secretions of both tissue-type and urokinase-type PA. The results of the present study suggest that polyamines may play a role in the regulation of PA synthesis and secretion and that this function can be modified under pathophysiological conditions affecting cellular and tissue levels of polyamines.
使用培养的牛主动脉内皮细胞评估了多胺腐胺（PUT）、亚精胺（SPD）和精胺（SPM）对纤溶酶原激活物（PA）和纤溶酶原激活物抑制剂（PAI）分泌的影响。所有三种多胺均以时间和剂量依赖性方式增强PA分泌，其效力顺序为SPM大于SPD大于PUT。如放线菌素D和环己酰亚胺抑制多胺诱导的PA分泌所证明，PA刺激需要RNA和蛋白质合成。多胺生物合成抑制剂甲基乙二醛双（胍基腙）（MGBG）和dl-（二氟甲基）鸟氨酸（DFMO）单独不影响基础或多胺诱导的PA分泌，但MGBG降低了PUT的作用除外。多胺处理的细胞增强了组织型和尿激酶型PA的分泌。本研究结果表明，多胺可能在PA合成和分泌的调节中起作用，并且在影响多胺细胞和组织水平的病理生理条件下，该功能可能会被改变。
