Sjögren A, Hillensjö T, Roos P, Hamberger L
Department of Obstetrics and Gynaecology, University of Göteborg, Sweden.
Hum Reprod. 1988 Jul;3(5):601-5. doi: 10.1093/oxfordjournals.humrep.a136753.
Human granulosa cells were isolated from preovulatory follicles during cycles stimulated with HMG-HCG or clomiphene-HMG-HCG or from unstimulated cycles. The cells were cultured for 6-8 days in medium M199 containing fetal calf serum under 5% CO2 in air. Highly purified human prolactin and human chorionic gonadotrophin were added alone or in combination to the cultures, and the content of steroids in the medium was measured every second day, utilizing conventional RIA techniques. In the presence of HCG the formation of progesterone (P) increased 3-5-fold over the control level with maximal effect after 4 days. In cells derived from clomiphene-HMG-HCG stimulated cycles, prolactin per se did not influence basal P formation but reduced the stimulatory effect of HCG. This was only seen in granulosa cells from follicles greater than 20 mm in diameter. In experiments with Forskolin, an adenylate cyclase activator, P formation was stimulated and the stimulation was counteracted by the concomitant presence of prolactin, indicating that prolactin did not interfere with the LH-HCG receptor. In cells from smaller follicles, or in cells from follicles aspirated from the natural cycle prior to the endogenous LH peak, P formation was stimulated by HCG but the addition of prolactin did not reduce this stimulatory effect. The results are discussed in relation to earlier reports on prolactin effects in vitro both on laboratory animals and human material.
人颗粒细胞取自用HMG-HCG或克罗米芬-HMG-HCG刺激的周期中的排卵前卵泡，或取自未受刺激的周期。将细胞在含胎牛血清的M199培养基中于5%二氧化碳的空气环境下培养6 - 8天。将高度纯化的人催乳素和人绒毛膜促性腺激素单独或联合添加到培养物中，每隔一天利用传统的放射免疫分析技术测量培养基中类固醇的含量。在有HCG存在的情况下，孕酮（P）的生成比对照水平增加3 - 5倍，4天后达到最大效应。在来自克罗米芬-HMG-HCG刺激周期的细胞中，催乳素本身并不影响基础P的生成，但会降低HCG的刺激作用。这仅在直径大于20毫米的卵泡的颗粒细胞中观察到。在用腺苷酸环化酶激活剂福斯可林进行的实验中，P的生成受到刺激，且这种刺激被同时存在的催乳素抵消，这表明催乳素不干扰LH-HCG受体。在来自较小卵泡的细胞中，或在内源性LH峰之前取自自然周期的卵泡的细胞中，HCG刺激P的生成，但添加催乳素并不会降低这种刺激作用。结合早期关于催乳素对实验动物和人类材料体外作用的报道对结果进行了讨论。
