Chen Zhi-Han, Wang Rui, Xie Jin, Ren Yu-Lan
College of Acupuncture-Moxibustion and Tuina, Chengdu University of TCM, Chengdu 610075, Sichuan Province, China.
Zhongguo Zhen Jiu. 2019 Aug 12;39(8):855-60. doi: 10.13703/j.0255-2930.2019.08.015.
To explore the action mechanism of acupoint selection along meridians to improve adenosine receptor in myocardial ischemia (MI) rats by comparing the effects of acupoint selection along meridians, acupoint selection at other meridians and non-acupoint on expression of adenosine receptor.
A total of 120 SD rats were randomly divided into a blank group, a sham operation group, a model group, an acupoint-selection-along-meridians (ASAM) group, an acupoint-selection-at-other-meridians (ASAOM) group and a non-acupoint group, 20 rats in each group. The model of MI was not made in the blank group; the left anterior descending coronary artery was not ligated after thoracotomy in the sham operation group; the model of MI was made but acupuncture was not given in the model group. After the model of MI was made, electroacupuncture (EA) was applied at "Neiguan" (PC 6) in the ASAM group, at "Hegu" (LI 4) in the ASAOM group, and at the area between the third and fourth metatarsal bone in the non-acupoint group. EA was given 20 min per treatment, once a day for 5 days. After treatment, the TTC staining was used to detect myocardial infarction, the Tunel method was used to detect cardiomyocyte apoptosis, and the immunohistochemistry was used to detect the expression of adenosine receptors A1, A2a and A2b.
Compared with the blank group and the sham operation group, the percentage of myocardial infarction and apoptotic rate of myocardial cells in the model group were increased significantly (<0.01). After EA treatment, compared with the model group, the percentage of myocardial infarction and apoptotic rate of myocardial cells in the ASAM group were decreased significantly (<0.01), and the expression levels of adenosine receptors A1, A2a and A2b were increased significantly (<0.01). The percentage of myocardial infarction and apoptotic rate of myocardial cells in the ASAM group were significantly lower than those in the ASAOM group and the non-acupoint group (<0.01), and the expression levels of adenosine receptors A1, A2a and A2b in the ASAM group were significantly higher than those in the ASAOM group and non-acupoint group (<0.01).
Compared with acupoint selection at other meridians or non-acupoints, acupoint selection along meridians can effectively regulate the expression of adenosine receptors A1, A2a and A2b, improve the condition of myocardial infarction, inhibit myocardial cell apoptosis, and consequently protect ischemic myocardium.
通过比较循经选穴、他经选穴及非经非穴对心肌缺血（MI）大鼠腺苷受体表达的影响，探讨循经选穴改善MI大鼠腺苷受体的作用机制。
将120只SD大鼠随机分为空白组、假手术组、模型组、循经选穴组（ASAM组）、他经选穴组（ASAOM组）和非经非穴组，每组20只。空白组不制作MI模型；假手术组开胸后不结扎左冠状动脉前降支；模型组制作MI模型但不给予针刺。制作MI模型后，ASAM组于“内关”（PC 6）行电针（EA），ASAOM组于“合谷”（LI 4）行EA，非经非穴组于第三、四跖骨间区域行EA。每次治疗EA持续20分钟，每天1次，共5天。治疗后，采用TTC染色检测心肌梗死情况，采用Tunel法检测心肌细胞凋亡情况，采用免疫组化法检测腺苷受体A1、A2a和A2b的表达。
与空白组和假手术组比较，模型组心肌梗死面积百分比及心肌细胞凋亡率显著升高（<0.01）。EA治疗后，与模型组比较，ASAM组心肌梗死面积百分比及心肌细胞凋亡率显著降低（<0.01），腺苷受体A1、A2a和A2b表达水平显著升高（<0.
