Universidade de Lisboa, Instituto Superior de Agronomia, Centro de Estudos Florestais, Lisbon, Portugal.
Universidade de Lisboa, Instituto Superior de Agronomia, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal.
Bioresour Technol. 2019 Nov;292:121936. doi: 10.1016/j.biortech.2019.121936. Epub 2019 Aug 1.
Spent olive pomace from the two-phase extraction system of virgin olive oil and olive pomace oil, is a major agro-industrial residue. Present study aimed at the valorization of residual olive pomace and stones (seeds) by hydrothermal treatment and enzymatic hydrolysis of glucans. Both residues contain lignin (31.2% and 42.1%), glucans (13.8% and 15.3%) and xylans (18.9% and 20.3%). After hydrothermal pretreatment (130 °C, 30 min; severity factor log R = 2.99), 65% and 75% of hemicelluloses (65% of xylan) were hydrolysed into xylo-oligosaccharides in pomace and stones, respectively. Cellulose and lignin were not substantially affected. Three commercial enzyme preparations, Saczyme Yield, Ultimase BWL 40 and Celluclast 1.5 L, were evaluated for saccharification of pomace or stones at three biomass loads (10, 20 and 30%, w/v). Saczyme and Ultimase were active with high solid loads (30%), reaching 80 and 90% of glucan conversion in pomace, and 40 and 55% in stones, respectively, after 5 h.
橄榄渣是初榨橄榄油和橄榄渣油双相提取系统的主要农业工业废料。本研究旨在对橄榄渣和橄榄核（种子）进行水热处理和酶解葡聚糖，实现其增值利用。两种残渣均含有木质素（31.2%和 42.1%）、葡聚糖（13.8%和 15.3%）和木聚糖（18.9%和 20.3%）。经过水热预处理（130°C，30 分钟；严重度因子 log R = 2.99）后，橄榄渣和橄榄核中的半纤维素分别有 65%和 75%（65%为木聚糖）水解成木二糖低聚糖。纤维素和木质素没有受到明显影响。三种商业酶制剂，Saczyme Yield、Ultimase BWL 40 和 Celluclast 1.5L，用于在三种生物质负载（10%、20%和 30%，w/v）下对橄榄渣或橄榄核进行糖化。Saczyme 和 Ultimase 在高固体负载（30%）下具有活性，在 5 小时后，在橄榄渣中达到 80%和 90%的葡聚糖转化率，在橄榄核中分别达到 40%和 55%。
