Takeda O, Yamasaki K, Kohda H, Yamashita A, Kurokawa T, Ishibashi S
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
J Pharmacobiodyn. 1988 May;11(5):377-80. doi: 10.1248/bpb1978.11.377.
Forskolin-activated adenylate cyclase activity in rat erythrocyte membranes was markedly inhibited by methanol in a concentration dependent manner. On the contrary, no change was observed in the basal cyclase activity in the presence of methanol. The methanol inhibition forskolin-activated cyclase activity was protected by the stimulation of Gs in the presence of NaF in a concentration dependent manner. These data indicate that low- and high-affinity binding sites of forskolin in rat erythrocyte membranes have different sensitivities to methanol.
福司可林激活的大鼠红细胞膜腺苷酸环化酶活性受到甲醇的显著抑制，且呈浓度依赖性。相反，在甲醇存在的情况下，基础环化酶活性未观察到变化。甲醇对福司可林激活的环化酶活性的抑制作用在氟化钠存在下通过刺激Gs呈浓度依赖性得到保护。这些数据表明，大鼠红细胞膜中福司可林的低亲和力和高亲和力结合位点对甲醇具有不同的敏感性。
