Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585, Japan.
Department of Applied Chemistry, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Colloids Surf B Biointerfaces. 2019 Nov 1;183:110370. doi: 10.1016/j.colsurfb.2019.110370. Epub 2019 Aug 2.
Thermoresponsive core-corona type nanoparticles were prepared exhibiting biomolecule recognition ability on their surfaces. These thermoresponsive nanoparticles were prepared from a poly(N-isopropylacrylamide) (PNIPAAm) macro-initiator and styrene (St) in a polar solvent via atom transfer radical dispersion polymerization. The PNIPAAm macro-initiator contains an alkyl halide and/or phthalimide group on the terminated group of the polymer chain, and thus, the grafting of PNIPAAm on the PSt core resulted in terminating phthalimide end groups. These terminal groups were utilized to immobilize biomolecule recognition units, and the dispersion stabilities of the nanoparticles were found to change in aqueous solution at room temperature due to alteration of the terminating PNIPAAm groups by the presence of biomolecules at different concentrations.
温敏核壳型纳米粒子被制备出来，其表面具有生物分子识别能力。这些温敏纳米粒子是通过原子转移自由基分散聚合，由聚（N-异丙基丙烯酰胺）（PNIPAAm）大分子引发剂和苯乙烯（St）在极性溶剂中制备的。PNIPAAm 大分子引发剂在聚合物链的末端基团上含有卤代烷基和/或邻苯二甲酰亚胺基团，因此，PNIPAAm 接枝到 PSt 核上导致邻苯二甲酰亚胺端基的终止。这些端基被用来固定生物分子识别单元，并且发现纳米粒子在水溶液中的分散稳定性会由于不同浓度的生物分子存在时，终止的 PNIPAAm 基团的变化而发生变化。
