Touré Aminata, Clemente Emily J, Ellis Peter J I, Mahadevaiah Shantha K, Ojarikre Obah A, Ball Penny A F, Reynard Louise, Loveland Kate L, Burgoyne Paul S, Affara Nabeel A
Division of Developmental Genetics, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK.
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
Genome Biol. 2019 Aug 9;20(1):160. doi: 10.1186/s13059-019-1779-z.
Following publication of the original article [1], the following error was reported: The actin control panel in Fig. 3 of this paper is reproduced from Fig. 7 of Touré et al, 2004 [2] by kind permission of the Genetics Society of America. Touré et al, 2004 used Northern blotting to show that the Y-linked genes Ssty1 and Ssty2 have reduced expression in a range of mouse genotypes with deletions on the Y chromosome long arm. This paper shows that two novel genes, Sly and Asty are also present on mouse Yq and have reduced expression in these deleted genotypes. A further companion paper was published in Human Molecular Genetics (Ellis et al, 2005 [3]) showing that X-linked genes are upregulated in the various deleted genotypes. Since two of the genotypes concerned are sterile and very hard to generate, all the Northern blot experiments in these papers were performed on a single membrane that was stripped and re-probed with a range of different X- and Y-linked genes. The same beta-actin loading control image thus necessarily applies to all the data presented, and was shown in all three papers. We regret that this was not mentioned appropriately in the Methods and figure legends at the time of publication.
在原始文章[1]发表后,发现了以下错误:本文图3中的肌动蛋白对照图经美国遗传学会许可,复制自图雷等人2004年的图7[2]。图雷等人2004年使用Northern印迹法表明,Y连锁基因Ssty1和Ssty2在一系列Y染色体长臂缺失的小鼠基因型中表达降低。本文表明,两个新基因Sly和Asty也存在于小鼠Yq上,并且在这些缺失的基因型中表达降低。另一篇相关的论文发表在《人类分子遗传学》(埃利斯等人,2005年[3])上,表明X连锁基因在各种缺失的基因型中上调。由于其中两种相关的基因型是不育的,并且很难产生,因此这些论文中的所有Northern印迹实验都是在一张膜上进行的,该膜经过剥离并用一系列不同的X和Y连锁基因重新检测。因此,相同的β-肌动蛋白上样对照图像必然适用于所有呈现的数据,并且在所有三篇论文中都有展示。我们很遗憾在发表时,方法和图注中没有对此进行适当说明。