Department of Pharmacology, College of Pharmacy, Beihua University, Jilin, Jilin, 132013, China.
Department of Molecular Biology, College of Laboratory Medicine, Beihua University, Jilin, Jilin, 132002, China.
J Pharmacol Sci. 2019 Jul;140(3):248-254. doi: 10.1016/j.jphs.2019.07.008. Epub 2019 Jul 24.
Schisandra chinensis is a hepatoprotective herb that has been used for centuries in China. Polysaccharide is one of the major active components in S. chinensis, which has been reported to improve liver injuries induced by carbon tetrachloride, alcohol, or high-fat diet. In this study, we observed the effects and corresponding mechanisms of the secondary component of Schisandra polysaccharide (acidic polysaccharide, SCAP) on a murine model of severe acute liver injury induced by acetaminophen (APAP). SCAP significantly decreased the serum alanine aminotransferase (ALT), aspartate aminotransferas (AST), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) levels, and was found to alleviate hepatic pathological alterations in the mouse model. Meanwhile, SCAP revealed a protective effects on the liver injury-related enzymes and factors, such as significantly diminished malondialdehyde (MDA) levels and glutathione (GSH) depletion, reduced ratio of B-cell lymphoma-2 (Bcl-2)-associated X protein (Bax)/Bcl-2, prohibited cleaved caspase-3 expression, and elevated the expression of p-AMPK, p-Akt, p-glycogen synthase kinase 3β (GSK 3β), nuclear factor erythroid 2-derived-like 2 (Nrf 2) and heme oxygenase-1 (HO-1) proteins in the liver tissues of the mouse model. In conclusion, we speculated that the protective activities of SCAP on the APAP-induced mouse model of acute liver injury might be related to its antioxidation, anti-inflammation and anti-apoptosis properties.
五味子是一种具有护肝作用的草药，在中国已有数百年的应用历史。多糖是五味子的主要活性成分之一，据报道，它可以改善四氯化碳、酒精或高脂肪饮食引起的肝损伤。在这项研究中，我们观察了五味子多糖（酸性多糖，SCAP）的次级成分对乙酰氨基酚（APAP）诱导的严重急性肝损伤小鼠模型的作用及相应机制。SCAP 显著降低了血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平，并且发现它可以减轻小鼠模型中的肝病理改变。同时，SCAP 对与肝损伤相关的酶和因子具有保护作用，如显著降低丙二醛（MDA）水平和谷胱甘肽（GSH）耗竭，降低 B 细胞淋巴瘤-2（Bcl-2）相关 X 蛋白（Bax）/Bcl-2 的比值，抑制半胱氨酸天冬氨酸蛋白酶-3（caspase-3）的表达，提高磷酸化腺苷酸活化蛋白激酶（p-AMPK）、磷酸化蛋白激酶 B（p-Akt）、磷酸化糖原合成酶激酶 3β（p-GSK 3β）、核因子红细胞 2 相关因子 2（Nrf 2）和血红素加氧酶-1（HO-1）蛋白在小鼠肝组织中的表达。总之，我们推测 SCAP 对 APAP 诱导的急性肝损伤小鼠模型的保护作用可能与其抗氧化、抗炎和抗凋亡特性有关。
