Ma Shengwei, Yuan Yang, Tao Ye, Jia Haiyan, Ma Zhengqiang
The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Genomics. 2020 Mar;112(2):1343-1350. doi: 10.1016/j.ygeno.2019.08.003. Epub 2019 Aug 8.
Lineage-specific genes (LSGs) are a set of genes in a given taxon without significant sequence similarity to genes and intergenic sequences of other taxa and are functional. The tribe Triticeae mainly includes species of different ploidy levels, such as staple food crops wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). This study is aimed at mining and characterizing the Triticeae-specific genes (TSGs) using expressed sequence data of wheat. A total of 3812 TSGs was identified and they were generally characterized by smaller size, fewer exons, shorter open reading frames and lower expression levels. Most TSGs were expressed with tissue preference and many of them were predominantly expressed in reproduction related tissues, especially in young stamen. Nearly one third of the TSGs were stress-responsive and inducible under abiotic and/or biotic stresses. A co-expression-based annotation supported the relevance of some TSGs with reproduction and stress responses, indicating their potential economic importance.
谱系特异性基因（LSGs）是特定分类群中的一组基因，与其他分类群的基因和基因间序列没有显著的序列相似性，但具有功能。小麦族主要包括不同倍性水平的物种，如主食作物小麦（Triticum aestivum L.）和大麦（Hordeum vulgare L.）。本研究旨在利用小麦的表达序列数据挖掘和鉴定小麦族特异性基因（TSGs）。共鉴定出3812个TSGs，它们通常具有较小的大小、较少的外显子、较短的开放阅读框和较低的表达水平。大多数TSGs具有组织偏好性表达，其中许多主要在与生殖相关的组织中表达，尤其是在幼嫩雄蕊中。近三分之一的TSGs对胁迫有响应，在非生物和/或生物胁迫下可被诱导。基于共表达的注释支持了一些TSGs与生殖和胁迫响应的相关性，表明它们具有潜在的经济重要性。
