Chechik B E, Minowada J
J Natl Cancer Inst. 1979 Sep;63(3):609-14. doi: 10.1093/jnci/63.3.609.
A competitive radioimmunoassay for a saline-soluble human thymus-leukemia-associated antigen (HThy-L) was applied for quantitation of this antigen in leukemia and normal hematopoietic cell lines. Highly increased quantities of HThy-L were detected in all T-cell leukemia lines tested, regardless of the presence or absence of receptors for sheep erythrocytes. This elevated level of HThy-L in combination with high terminal deoxynucleotidyl transferase and adenosine deaminase activities and the presence of a T-lymphocyte-specific surface antigen appear to represent stable phenotypic characteristics of T-cell lines. Most normal B-cell lines had low quantities of HTy-L. The level of HThy-L was slightly elevated in a considerable number of lymphoma B-cell lines and in all non-T, non-B leukemia cell lines tested. No relationship existed between quantities of HThy-L and an expression of different surface immunoglobulin isotypes in B-cell lines. Low quantities of HThy-L were detected in leukemia myeloid and myeloma cell lines as well as in B-cell leukemia lines originating from patients with B-cells acute lymphoblastic leukemia. Apparently, the increased quantities of HThy-L in T-cell leukemia lines may be related to certain stages of T-cell differentiation at which leukemia cell transformation occurs.
应用一种针对盐溶性人胸腺白血病相关抗原（HThy-L）的竞争性放射免疫测定法，对白血病和正常造血细胞系中的该抗原进行定量分析。在所有检测的T细胞白血病系中均检测到HThy-L的量显著增加，无论是否存在绵羊红细胞受体。HThy-L的这种升高水平，与高末端脱氧核苷酸转移酶和腺苷脱氨酶活性以及T淋巴细胞特异性表面抗原的存在，似乎代表了T细胞系稳定的表型特征。大多数正常B细胞系的HThy-L含量较低。在相当数量的淋巴瘤B细胞系以及所有检测的非T、非B白血病细胞系中，HThy-L水平略有升高。B细胞系中HThy-L的量与不同表面免疫球蛋白同种型的表达之间不存在关联。在白血病髓系和骨髓瘤细胞系以及源自B细胞急性淋巴细胞白血病患者的B细胞白血病系中检测到低量的HThy-L。显然，T细胞白血病系中HThy-L量的增加可能与白血病细胞发生转化的T细胞分化的某些阶段有关。
