Hendricks Robert, Baker Dana, Brumm Jochen, Davancaze Teresa, Harp Chris, Herman Ann, Büdingen H-Christian von, Townsend Michael, Fischer Saloumeh K
Department of BioAnalytical Sciences, Genentech, South San Francisco, CA 94080, USA.
Department of Biostatistics, Genentech, South San Francisco, CA 94080, USA.
Bioanalysis. 2019 Aug;11(15):1405-1418. doi: 10.4155/bio-2019-0163. Epub 2019 Aug 12.
Neurofilament light (NfL) chain is an established cerebrospinal fluid (CSF) biomarker for neuroaxonal injury. The highly sensitive Quanterix Simoa™ platform is evaluated for NfL measurement in both CSF and blood. There is a need to link historical ELISA data that use bovine NfL to that of Simoa using a recombinant human (rhuman) NfL standard. The Simoa NF-light Advantage Kit was validated for CSF and qualified for serum and plasma, using both rhuman and bovine NfL calibrators. Matched CSF, serum and plasma samples from 112 multiple sclerosis patients were analyzed using both calibrators. In multiple sclerosis, there is a good correlation between blood and CSF NfL levels. A conversion factor of approximately 5:1 was established between bovine and rhuman NfL calibrators.
神经丝轻链（NfL）是一种公认的用于神经轴突损伤的脑脊液（CSF）生物标志物。对高灵敏度的Quanterix Simoa™平台进行了评估，以用于脑脊液和血液中NfL的测量。需要使用重组人（rhuman）NfL标准品，将使用牛NfL的历史ELISA数据与Simoa的数据联系起来。Simoa NF-light优势试剂盒使用rhuman和牛NfL校准品，对脑脊液进行了验证，对血清和血浆进行了鉴定。使用两种校准品对112例多发性硬化症患者的配对脑脊液、血清和血浆样本进行了分析。在多发性硬化症中，血液和脑脊液NfL水平之间存在良好的相关性。在牛和rhuman NfL校准品之间建立了约5:1的转换因子。
